ArticleNature protocols2009
Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression.
Article in Nature protocols, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Silencing Multiple Crustacean Hyperglycaemic Hormone-Encoding Genes in the Redclaw CrayfishInternational journal of molecular sciences · 2024Article
- Lipid Nanoparticles for Nucleic Acid Delivery to Endothelial Cells.Pharmaceutical research · 2023Review
- ASB6 Promotes the Stemness Properties and Sustains Metastatic Potential of Oral Squamous Cell Carcinoma Cells by Attenuating ER Stress.International journal of biological sciences · 2019Article
- IGF-1 receptor regulates dynamic changes in neuronal polarity during cerebral cortical migration.Scientific reports · 2017Article
- Knockdown and replacement therapy mediated by artificial mirtrons in spinocerebellar ataxia 7.Nucleic acids research · 2017Article
- Reconstitution of calcium-mediated exocytosis of dense-core vesicles.Science advances · 2017Article
- A multi-shRNA vector enhances the silencing efficiency of exogenous and endogenous genes in human cells.Oncology letters · 2017Article
- SUMOylated MAFB promotes colorectal cancer tumorigenesis.Oncotarget · 2016Article
- Subunit-selective N-Methyl-d-aspartate (NMDA) Receptor Signaling through Brefeldin A-resistant Arf Guanine Nucleotide Exchange Factors BRAG1 and BRAG2 during Synapse Maturation.The Journal of biological chemistry · 2016Article
- Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana.Plant methods · 2016Article
- DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.BMC genomics · 2015Article
- Viral vector-based improvement of optic nerve regeneration: characterization of individual axons' growth patterns and synaptogenesis in a visual target.Gene therapy · 2015Article
- The 15kDa selenoprotein and thioredoxin reductase 1 promote colon cancer by different pathways.PloS one · 2015Article
- The transcription factor Cux1 in cerebellar granule cell development and medulloblastoma pathogenesis.Cerebellum (London, England) · 2014Article
- SUMO1 modification stabilizes CDK6 protein and drives the cell cycle and glioblastoma progression.Nature communications · 2014Article
- Article
- Alteration of thioredoxin reductase 1 levels in elucidating cancer etiology.Methods in enzymology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Small hairpin RNA (shRNA) is a powerful tool for inhibiting gene expression. One limitation has been that this technique has been used primarily to target a single gene. This protocol expands upon previous methods by describing a knockdown vector that facilitates cloning of multiple shRNAs; this allows targeted knockdown of more than one gene or of a single gene that may otherwise be difficult to knockdown using a single shRNA. The targeted gene(s) can be readily re-expressed by transfecting knockdown cells with a knock-in vector, containing an shRNA-refractive cDNA that will express the protein-of-interest even in the presence of shRNAs. The constructed knockdown and knock-in vectors can be easily used concurrently to assess possible interrelationships between genes, the effects of gene loss on cell function and/or their restoration by replacing targeted genes one at a time. The entire knockdown or knock-in procedure can be completed in approximately 3-4 months.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.