ArticleStem cells (Dayton, Ohio)2009
Bioluminescent imaging demonstrates that transplanted human embryonic stem cell-derived CD34(+) cells preferentially develop into endothelial cells.
Article in Stem cells (Dayton, Ohio), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Non-invasive Reporter Gene Imaging of Cell Therapies, including T Cells and Stem Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Review
- Aryl hydrocarbon receptor inhibition promotes hematolymphoid development from human pluripotent stem cells.Blood · 2017Article
- Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence.Nature communications · 2017Article
- A synthetic three-dimensional niche system facilitates generation of functional hematopoietic cells from human-induced pluripotent stem cells.Journal of hematology & oncology · 2016Article
- Modulation of human allogeneic and syngeneic pluripotent stem cells and immunological implications for transplantation.Transplantation reviews (Orlando, Fla.) · 2016Review
- Efficient generation of megakaryocytes from human induced pluripotent stem cells using food and drug administration-approved pharmacological reagents.Stem cells translational medicine · 2015Article
- Article
- Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells.Experimental hematology · 2014Article
- Functional assessment of hematopoietic niche cells derived from human embryonic stem cells.Stem cells and development · 2014Article
- Humanized-BLT mouse model of Kaposi's sarcoma-associated herpesvirus infection.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Review
- Intraperitoneal delivery of human natural killer cells for treatment of ovarian cancer in a mouse xenograft model.Cytotherapy · 2013Article
- Engineered human embryonic stem cell-derived lymphocytes to study in vivo trafficking and immunotherapy.Stem cells and development · 2013Article
- Development, expansion, and in vivo monitoring of human NK cells from human embryonic stem cells (hESCs) and and induced pluripotent stem cells (iPSCs).Journal of visualized experiments : JoVE · 2013Article
- Clinical-scale derivation of natural killer cells from human pluripotent stem cells for cancer therapy.Stem cells translational medicine · 2013Article
- In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells.Blood · 2013Article
- Humanized mice for immune system investigation: progress, promise and challenges.Nature reviews. Immunology · 2012Review
- Review
- Immunity of embryonic stem cell-derived hematopoietic progenitor cells.Seminars in immunopathology · 2011Review
- Human pluripotent stem cells produce natural killer cells that mediate anti-HIV-1 activity by utilizing diverse cellular mechanisms.Journal of virology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Human embryonic stem cells (hESCs) provide an important resource for novel regenerative medicine therapies and have been used to derive diverse cell populations, including hematopoietic and endothelial cells. However, it remains a challenge to achieve significant engraftment of hESC-derived blood cells when transplanted into animal models. To better understand mechanisms that enhance or limit the in vivo developmental potential of hESC-derived cells, we utilized hESCs that express firefly luciferase (luc) to allow noninvasive, real-time bioluminescent imaging of hESC-derived CD34(+) cells transplanted into the liver of neonatal immunodeficient mice. Serial imaging demonstrated stable engraftment and expansion of the luc(+) hESC-derived cells in vivo over several months. While we found that these hESC-derived CD34(+) cells have bipotential ability to generate both hematopoietic and endothelial lineages in vitro, these studies demonstrate preferential differentiation into endothelial cells in vivo, with only low levels of hematopoietic cell engraftment. Therefore, these studies reveal key differences in the developmental potential of hESC-derived cells using in vitro and in vivo analyses. Although transplanted hESC-derived CD34(+) cells are well-suited for revascularization therapies, additional measures are needed to provide higher levels of long-term hematopoietic engraftment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.