ArticleMolecular biology of the cell2009
Knockdown of the Drosophila GTPase nucleostemin 1 impairs large ribosomal subunit biogenesis, cell growth, and midgut precursor cell maintenance.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Loss of Drosophila nucleostemin 1 disrupts ribosomal protein homeostasis and rRNA processing to trigger apoptosis via the Xrp1/Irbp18 complex.Cell death discovery · 2026Article
- Hierarchical interactions between nucleolar and heterochromatin condensates are mediated by a dual-affinity protein.Nature cell biology · 2025Article
- GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells.Medical oncology (Northwood, London, England) · 2023Article
- Ribosome biogenesis and function in development and disease.Development (Cambridge, England) · 2023Review
- NOC1 is a direct MYC target, and its protein interactome dissects its activity in controlling nucleolar function.Frontiers in cell and developmental biology · 2023Article
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- The roles of jim lovell and uninflatable in different endopolyploid larval tissues of Drosophila melanogaster.PloS one · 2020Article
- Systematic discovery of genetic modulation by Jumonji histone demethylases in Drosophila.Scientific reports · 2017Article
- Loss of Drosophila nucleostemin 2 (NS2) blocks nucleolar release of the 60S subunit leading to ribosome stress.Chromosoma · 2017Article
- Independent active and thermodynamic processes govern the nucleolus assembly in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cellular and Molecular Preconditions for Retinal Pigment Epithelium (RPE) Natural Reprogramming during Retinal Regeneration in Urodela.Biomedicines · 2016Review
- Emerging roles of nucleolar and ribosomal proteins in cancer, development, and aging.Cellular and molecular life sciences : CMLS · 2015Review
- Transgenerational inheritance of diet-induced genome rearrangements in Drosophila.PLoS genetics · 2015Article
- Turning a new page on nucleostemin and self-renewal.Journal of cell science · 2014Article
- Stressing on the nucleolus in cardiovascular disease.Biochimica et biophysica acta · 2014Review
- Nucleostemin and GNL3L exercise distinct functions in genome protection and ribosome synthesis, respectively.Journal of cell science · 2014Article
- Drosophila mbm is a nucleolar myc and casein kinase 2 target required for ribosome biogenesis and cell growth of central brain neuroblasts.Molecular and cellular biology · 2014Article
- Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Translational profiling of clock cells reveals circadianly synchronized protein synthesis.PLoS biology · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian nucleostemin (NS) is a nucleolar guanosine triphosphate-binding protein implicated in cell cycle progression, stem cell proliferation, and ribosome assembly. Drosophila melanogaster contains a four-member nucleostemin family (NS1-4). NS1 is the closest orthologue to human NS; it shares 33% identity and 67% similarity with human NS. We show that NS1 has intrinsic GTPase and ATPase activity and that it is present within nucleoli of most larval and adult cells. Endogenous NS1 and lightly expressed green fluorescent protein (GFP)-NS1 enrich within the nucleolar granular regions as expected, whereas overexpressed GFP-NS1 localized throughout the nucleolus and nucleoplasm, and to several transcriptionally active interbands of polytene chromosomes. Severe overexpression correlated with the appearance of melanotic tumors and larval/pupal lethality. Depletion of 60% of NS1 transcripts also lead to larval and pupal lethality. NS1 protein depletion>95 correlated with the loss of imaginal island (precursor) cells in the larval midgut and to an apparent block in the nucleolar release of large ribosomal subunits in terminally differentiated larval midgut polyploid cells. Ultrastructural examination of larval Malpighian tubule cells depleted for NS1 showed a loss of cytoplasmic ribosomes and a concomitant appearance of cytoplasmic preautophagosomes and lysosomes. We interpret the appearance of these structures as indicators of cell stress response.
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