ArticleMolecular biology of the cell2009
Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 53 citations in OpenAlex.
- Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.iScience · 2024Article
- Generation and characterization of conditional yeast mutants affecting each of the 2 essential functions of the scaffolding proteins Boi1/2 and Bem1.G3 (Bethesda, Md.) · 2022Article
- Negative control of cytokinesis by stress-activated MAPK signaling.Current genetics · 2021Review
- Eng2, a new player involved in feedback loop regulation of Cdc42 activity in fission yeast.Scientific reports · 2021Article
- Review
- Rho Family GTPases in Fission Yeast Cytokinesis.Communicative & integrative biology · 2019Review
- Evolutionary dynamics in the fungal polarization network, a mechanistic perspective.Biophysical reviews · 2017Review
- Internetwork competition for monomers governs actin cytoskeleton organization.Nature reviews. Molecular cell biology · 2016Article
- Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.Molecular biology of the cell · 2016Article
- Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.The Journal of biological chemistry · 2014Article
- Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.PLoS genetics · 2013Article
- The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.PloS one · 2013Article
- Myosin Vs organize actin cables in fission yeast.Molecular biology of the cell · 2012Article
- Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.Traffic (Copenhagen, Denmark) · 2012Article
- Cdc42p regulation of the yeast formin Bni1p mediated by the effector Gic2p.Molecular biology of the cell · 2012Article
- Cdc42 regulation of polarized traffic in fission yeast.Communicative & integrative biology · 2012Article
- Article
- Spatial control of Cdc42 activation determines cell width in fission yeast.Molecular biology of the cell · 2011Article
- Three's company: the fission yeast actin cytoskeleton.Trends in cell biology · 2011Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rho GTPases regulate the actin cytoskeleton in all eukaryotes. Fission yeast Cdc42 is involved in actin cable assembly and formin For3 regulation. We isolated cdc42-879 as a thermosensitive strain with actin cable and For3 localization defects. In a multicopy suppressor screening, we identified pob1(+) as suppressor of cdc42-879 thermosensitivity. Pob1 overexpression also partially restores actin cables and localization of For3 in the mutant strain. Pob1 interacts with Cdc42 and this GTPase regulates Pob1 localization and/or stability. The C-terminal pleckstrin homology (PH) domain of Pob1 is required for Cdc42 binding. Pob1 also binds to For3 through its N-terminal sterile alpha motif (SAM) domain and contributes to the formin localization at the cell tips. The previously described pob1-664 mutant strain (Mol. Biol. Cell. 10, 2745-2757, 1999), which carries a mutation in the PH domain, as well as pob1 mutant strains in which Pob1 lacks the N-terminal region (pob1DeltaN) or the SAM domain (pob1DeltaSAM), have cytoskeletal defects similar to that of cdc42-879 cells. Expression of constitutively active For3DAD* partially restores actin organization in cdc42-879, pob1-664, pob1DeltaN, and pob1DeltaSAM. Therefore, we propose that Pob1 is required for For3 localization to the tips and facilitates Cdc42-mediated relief of For3 autoinhibition to stimulate actin cable formation.
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