ArticlePlant physiology2009
Nitric reductase-dependent nitric oxide production is involved in cold acclimation and freezing tolerance in Arabidopsis.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.
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140 citing papers in PubMed, 520 citations in OpenAlex.
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- Cerium oxide nanoparticles enhance rice productivity by modulating nitric oxide signaling, carbon metabolism, and potassium homeostasis under chromium-induced environmental stress.Frontiers in plant science · 2026Article
- The Central Role of GSNOR: Decoding Nitric Oxide Signaling for Crop Stress Tolerance.International journal of molecular sciences · 2025Review
- Phenolic Compounds Enhance Aluminum Tolerance in Chinese Fir (Plants (Basel, Switzerland) · 2025Article
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- Regulation of NO-Generating System Activity in Cucumber Root Response to Cold.International journal of molecular sciences · 2025Article
- Unraveling the signaling pathways of plant cold stress: current insights and future directions.Frontiers in plant science · 2025Review
- Nitric oxide-mediated modulation of reproductive resilience under cold stress in chickpea.Frontiers in plant science · 2025Article
- Dynamic changes in the transcriptome of tropical region-originated king grasses in response to cold stress.Frontiers in plant science · 2025Article
- Nitric Oxide Pre-Treatment Advances Bulblet Dormancy Release by Mediating Metabolic Changes inInternational journal of molecular sciences · 2024Article
- Transcriptomic analyses provide molecular insight into the cold stress response of cold-tolerant alfalfa.BMC plant biology · 2024Article
- Dynamic Arabidopsis P5CS filament facilitates substrate channelling.Nature plants · 2024Article
- Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation duringPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Impacts of fertilization methods onFrontiers in plant science · 2024Article
- Melatonin activates the OsbZIP79-OsABI5 module that orchestrates nitrogen and ROS homeostasis to alleviate nitrogen-limitation stress in rice.Plant communications · 2023Article
- Exogenous Putrescine Modulates Nitrate Reductase-Dependent NO Production in Cucumber Seedlings Subjected to Salt Stress.Metabolites · 2023Article
- Multiple Ways of Nitric Oxide Production in Plants and Its Functional Activity under Abiotic Stress Conditions.International journal of molecular sciences · 2023Review
- Nitric Oxide Regulates Seed Germination by Integrating Multiple Signalling Pathways.International journal of molecular sciences · 2023Review
80 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) is an important signaling molecule involved in many physiological processes in plants. We evaluated the role of NO in cold acclimation and freezing tolerance using Arabidopsis (Arabidopsis thaliana) wild type and mutants nia1nia2 (for nitrate reductase [NR]-defective double mutant) and Atnoa1/rif1 (for nitric oxide associated1/resistant to inhibition by fosmidomycin1) that exhibit defects in NR and reduced NO production, respectively. Cold acclimation induced an increase in endogenous NO production in wild-type and Atnoa1/rif1 leaves, while endogenous NO level in nia1nia2 leaves was lower than in wild-type ones and was little changed during cold acclimation. Cold acclimation stimulated NR activity and induced up-regulation of NIA1 gene expression. In contrast, cold acclimation reduced the quantity of NOA1/RIF1 protein and inhibited NO synthase (NOS) activity. These results indicate that up-regulation of NR-dependent NO synthesis underpins cold acclimation-induced NO production. Seedlings of nia1nia2 were less tolerant to freezing than wild-type plants. Pharmacological studies using NR inhibitor, NO scavenger, and NO donor showed that NR-dependent NO level was positively correlated with freezing tolerance. Furthermore, cold acclimation up- and down-regulated expression of P5CS1 and ProDH genes, respectively, resulting in enhanced accumulation of proline (Pro) in wild-type plants. The stimulation of Pro accumulation by cold acclimation was reduced by NR inhibitor and NO scavenger, while Pro accumulation by cold acclimation was not affected by the NOS inhibitor. In contrast to wild-type plants, cold acclimation up-regulated ProDH gene expression in nia1nia2 plants, leading to less accumulation in nia1nia2 plants than in wild-type plants. These findings demonstrate that NR-dependent NO production plays an important role in cold acclimation-induced increase in freezing tolerance by modulating Pro accumulation in Arabidopsis.
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