ArticleJournal of virology2009
Mutational analysis of the Bunyamwera orthobunyavirus nucleocapsid protein gene.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
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- Baseline mapping of Oropouche virology, epidemiology, therapeutics, and vaccine research and development.NPJ vaccines · 2022Review
- Structural and functional similarities in bunyaviruses: Perspectives for pan-bunya antivirals.Reviews in medical virology · 2019Review
- Severe Fever With Thrombocytopenia Syndrome Virus-Induced Macrophage Differentiation Is Regulated by miR-146.Frontiers in immunology · 2019Article
- Mutational analysis of Rift Valley fever phlebovirus nucleocapsid protein indicates novel conserved, functional amino acids.PLoS neglected tropical diseases · 2017Article
- Isolation of a novel orthobunyavirus from bat flies (Eucampsipoda africana).The Journal of general virology · 2017Article
- RNA Encapsidation and Packaging in the Phleboviruses.Viruses · 2016Review
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- Article
- Evolution of the Bunyamwera virus polymerase to accommodate deletions within genomic untranslated region sequences.Journal of virology · 2015Article
- Temperature-sensitive mutations for live-attenuated Rift Valley fever vaccines: implications from other RNA viruses.Frontiers in microbiology · 2015Review
- Article
- Orthobunyaviruses: recent genetic and structural insights.Nature reviews. Microbiology · 2014Review
- Probing bunyavirus N protein oligomerisation using mass spectrometry.Rapid communications in mass spectrometry : RCM · 2014Article
- Genomic characterization of group C Orthobunyavirus reference strains and recent South American clinical isolates.PloS one · 2014Article
- Systems to establish bunyavirus genome replication in the absence of transcription.Journal of virology · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The bunyavirus nucleocapsid protein, N, is a multifunctional protein that encapsidates each of the three negative-sense genome segments to form ribonucleoprotein complexes that are the functional templates for viral transcription and replication. In addition, N protein molecules interact with themselves to form oligomers, with the viral L (RNA polymerase) protein, with the carboxy-terminal regions of either or both of the virion glycoproteins, and probably also with host cell proteins. Bunyamwera virus (BUNV), the prototype bunyavirus, encodes an N protein of 233 amino acids in length. To learn more about the roles of individual amino acids in the different interactions of N, we performed a wide-scale mutagenic analysis of the protein, and 110 single-point mutants were obtained. When the mutants were employed in a minireplicon assay to examine their effects on viral RNA synthesis, a wide range of activities compared to those of wild-type N protein were observed; changes at nine amino acid positions resulted in severely impaired RNA synthesis. Seventy-seven mutant clones were selected for use in the bunyavirus reverse genetics system, and 57 viable recombinant viruses were recovered. The recombinant viruses displayed a range of plaque sizes and titers in cell culture (from approximately 10(3) to 10(8) PFU/ml), and a number of viruses were shown to be temperature sensitive. Different assays were applied to determine why 20 mutant N proteins could not be recovered into infectious virus. Based on these results, a preliminary domain map of the BUNV N protein is proposed.
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