ArticleMolecular microbiology2009
The halophilic alkalithermophile Natranaerobius thermophilus adapts to multiple environmental extremes using a large repertoire of Na(K)/H antiporters.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 76 citations in OpenAlex.
- Alkaliphilic microbial cellulases: sources, structural insights, and bioengineering approaches for industrial applications.Archives of microbiology · 2026Review
- HadS, a membrane-associated 2-haloacid dehalogenase from the moderate halophileFrontiers in microbiology · 2026Article
- Natranaerobius thermophilus: an anaerobic, polyextremophilic microorganism with unique properties and adaptive mechanisms.Extremophiles : life under extreme conditions · 2025Review
- Diet-Microbiota-Host Interactions in Regulation of Cardiometabolic Homeostasis: Emerging Mechanisms and Therapeutic Potential.Microbiota and host · 2025Article
- Novel NhaC NaPlant molecular biology · 2024Article
- The polyextremophileApplied and environmental microbiology · 2024Article
- Article
- Halomonas salinarum sp. nov., a moderately halophilic bacterium isolated from saline soil in Yingkou, China.Archives of microbiology · 2022Article
- Highly integrated adaptive mechanisms in Spiribacter halalkaliphilus, a bacterium abundant in Chinese soda-saline lakes.Environmental microbiology · 2021Article
- Functional Characterization of MultipleInternational journal of molecular sciences · 2021Article
- Natranaerofaba carboxydovora gen. nov., sp. nov., an extremely haloalkaliphilic CO-utilizing acetogen from a hypersaline soda lake representing a novel deep phylogenetic lineage in the class 'Natranaerobiia'.Environmental microbiology · 2021Article
- The complete genome sequence of Rhodobaca barguzinensis alga05 (DSM 19920) documents its adaptation for life in soda lakes.Extremophiles : life under extreme conditions · 2018Article
- Identification of osmoadaptive strategies in the halophile, heterotrophic ciliate Schmidingerothrix salinarum.PLoS biology · 2018Article
- An Uncharacterized Major Facilitator Superfamily Transporter FromFrontiers in microbiology · 2018Article
- Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein FromFrontiers in microbiology · 2018Article
- Functional Role of MrpA in the MrpABCDEFG Na+/H+ Antiporter Complex from the Archaeon Methanosarcina acetivorans.Journal of bacteriology · 2017Article
- Mrp Antiporters Have Important Roles in Diverse Bacteria and Archaea.Frontiers in microbiology · 2017Review
- Alkaline Response of a Halotolerant Alkaliphilic Halomonas Strain and Functional Diversity of Its Na+(K+)/H+ Antiporters.The Journal of biological chemistry · 2016Article
- Alkaliphilic Bacteria with Impact on Industrial Applications, Concepts of Early Life Forms, and Bioenergetics of ATP Synthesis.Frontiers in bioengineering and biotechnology · 2015Review
- Microbial diversity and biogeochemical cycling in soda lakes.Extremophiles : life under extreme conditions · 2014Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natranaerobius thermophilus is an unusual extremophile because it is halophilic, alkaliphilic and thermophilic, growing optimally at 3.5 M Na(+), pH(55 degrees C) 9.5 and 53 degrees C. Mechanisms enabling this tripartite lifestyle are essential for understanding how microorganisms grow under inhospitable conditions, but remain unknown, particularly in extremophiles growing under multiple extremes. We report on the response of N. thermophilus to external pH at high salt and elevated temperature and identify mechanisms responsible for this adaptation. N. thermophilus exhibited cytoplasm acidification, maintaining an unanticipated transmembrane pH gradient of 1 unit over the entire extracellular pH range for growth. N. thermophilus uses two distinct mechanisms for cytoplasm acidification. At extracellular pH values at and below the optimum, N. thermophilus utilizes at least eight electrogenic Na(+)(K(+))/H(+) antiporters for cytoplasm acidification. Characterization of these antiporters in antiporter-deficient Escherichia coli KNabc showed overlapping pH profiles (pH 7.8-10.0) and Na(+) concentrations for activity (K(0.5) values 1.0-4.4 mM), properties that correlate with intracellular conditions of N. thermophilus. As the extracellular pH increases beyond the optimum, electrogenic antiport activity ceases, and cytoplasm acidification is achieved by energy-independent physiochemical effects (cytoplasmic buffering) potentially mediated by an acidic proteome. The combination of these strategies allows N. thermophilus to grow over a range of extracellular pH and Na(+) concentrations and protect biomolecules under multiple extreme conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.