ArticleProceedings of the National Academy of Sciences of the United States of America2009
Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 34 citations in OpenAlex.
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- Genetic analysis of the Drosophila ESCRT-III complex protein, VPS24, reveals a novel function in lysosome homeostasis.PloS one · 2021Article
- Bioinformatics analysis of prognostic value and prospective pathway signal of miR-30a in ovarian cancer.Journal of ovarian research · 2020Article
- Small heat shock proteins mediate cell-autonomous and -nonautonomous protection in a Drosophila model for environmental-stress-induced degeneration.Disease models & mechanisms · 2016Article
- Transmission, Development, and Plasticity of Synapses.Genetics · 2015Review
- The Neurexin/N-Ethylmaleimide-sensitive Factor (NSF) Interaction Regulates Short Term Synaptic Depression.The Journal of biological chemistry · 2015Article
- The destructive effect of botulinum neurotoxins on the SNARE protein: SNAP-25 and synaptic membrane fusion.PeerJ · 2015Article
- Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.PloS one · 2015Article
- A Distinct Perisynaptic Glial Cell Type Forms Tripartite Neuromuscular Synapses in the Drosophila Adult.PloS one · 2015Article
- Short-term synaptic depression is topographically distributed in the cochlear nucleus of the chicken.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Drosophila SNAP-29 is an essential SNARE that binds multiple proteins involved in membrane traffic.PloS one · 2014Article
- Expression of synaptosomal-associated protein-25 in the rat brain after subarachnoid hemorrhage.Neural regeneration research · 2013Article
- Molecular mechanisms of COMPLEXIN fusion clamp function in synaptic exocytosis revealed in a new Drosophila mutant.Molecular and cellular neurosciences · 2013Article
- Fast neurotransmitter release regulated by the endocytic scaffold intersectin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dance of the SNAREs: assembly and rearrangements detected with FRET at neuronal synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Sustaining rapid vesicular release at active zones: potential roles for vesicle tethering.Trends in neurosciences · 2013Review
- Genetic analysis in Drosophila reveals a role for the mitochondrial protein p32 in synaptic transmission.G3 (Bethesda, Md.) · 2012Article
- Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).Biochimica et biophysica acta · 2012Review
- The DISABLED protein functions in CLATHRIN-mediated synaptic vesicle endocytosis and exoendocytic coupling at the active zone.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Activity-dependent interactions of NSF and SNAP at living synapses.Molecular and cellular neurosciences · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current models of synaptic vesicle trafficking implicate a core complex of proteins comprised of N-ethylmaleimide-sensitive factor (NSF), soluble NSF attachment proteins (SNAPs), and SNAREs in synaptic vesicle fusion and neurotransmitter release. Despite this progress, major challenges remain in establishing the in vivo functions of these proteins and their roles in determining the physiological properties of synapses. The present study employs glutamatergic adult neuromuscular synapses of Drosophila, which exhibit conserved properties of short-term synaptic plasticity with respect to mammalian glutamatergic synapses, to address these issues through genetic analysis. Our findings establish an in vivo role for SNAP-25 in synaptic vesicle priming, and support a zippering model of SNARE function in this process. Moreover, these studies define the contribution of SNAP-25-dependent vesicle priming to the detailed properties of short-term depression elicited by paired-pulse (PP) and train stimulation. In contrast, NSF is shown here not to be required for WT PP depression, but to be critical for maintaining neurotransmitter release during sustained stimulation. In keeping with this role, disruption of NSF function results in activity-dependent redistribution of the t-SNARE proteins, SYNTAXIN and SNAP-25, away from neurotransmitter release sites (active zones). These findings support a role for NSF in replenishing active zone t-SNAREs for subsequent vesicle priming, and provide new insight into the spatial organization of SNARE protein cycling during synaptic activity. Together, the results reported here establish in vivo contributions of SNAP-25 and NSF to synaptic vesicle trafficking and define molecular mechanisms determining conserved functional properties of short-term depression.
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