ArticleProceedings of the National Academy of Sciences of the United States of America2009
Nonlinear electrophoretic response yields a unique parameter for separation of biomolecules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02171286 (The Oncopanel Pilot), which is not on this map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Oncopanel Pilot (TOP) Study
Who cites it
19 citing papers in PubMed.
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- An Integrated Strategy for Analyzing the Complete Complex Integrated Structure of Maize MON810 and Identification of an SNP in External Insertion Sequences.Plants (Basel, Switzerland) · 2024Article
- Detection of Circulating Tumor DNA in Plasma Using Targeted Sequencing.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Limitations and opportunities of technologies for the analysis of cell-free DNA in cancer diagnostics.Nature biomedical engineering · 2022Review
- Selective multiplexed enrichment for the detection and quantitation of low-fraction DNA variants via low-depth sequencing.Nature biomedical engineering · 2021Article
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- Exploiting biased reptation for continuous flow preparative DNA fractionation in a versatile microfluidic platform.Microsystems & nanoengineering · 2017Article
- Multiplexed enrichment of rare DNA variants via sequence-selective and temperature-robust amplification.Nature biomedical engineering · 2017Article
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- Characterization of a cold-active and salt tolerant esterase identified by functional screening of Arctic metagenomic libraries.BMC biochemistry · 2016Article
- Simulation-guided DNA probe design for consistently ultraspecific hybridization.Nature chemistry · 2015Article
- Current and future resources for functional metagenomics.Frontiers in microbiology · 2015Article
- Metagenomic small molecule discovery methods.Current opinion in microbiology · 2014Article
- Effective removal of co-purified inhibitors from extracted DNA samples using synchronous coefficient of drag alteration (SCODA) technology.International journal of legal medicine · 2013Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- The great screen anomaly--a new frontier in product discovery through functional metagenomics.Applied microbiology and biotechnology · 2012Review
- The potential of TaqMan Array Cards for detection of multiple biological agents by real-time PCR.PloS one · 2012Article
- Winnowing DNA for rare sequences: highly specific sequence and methylation based enrichment.PloS one · 2012Article
- Size Does Matter: Application-driven Approaches for Soil Metagenomics.Soil biology & biochemistry · 2010Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We demonstrate a unique parameter for biomolecule separation that results from the nonlinear response of long, charged polymers to electrophoretic fields and apply it to extraction and concentration of nucleic acids from samples that perform poorly under conventional methods. Our method is based on superposition of synchronous, time-varying electrophoretic fields, which can generate net drift of charged molecules even when the time-averaged molecule displacement generated by each field individually is zero. Such drift can only occur for molecules, such as DNA, whose motive response to electrophoretic fields is nonlinear. Consequently, we are able to concentrate DNA while rejecting high concentrations of contaminants. We demonstrate one application of this method by extracting DNA from challenging samples originating in the Athabasca oil sands.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.