ArticleProceedings of the National Academy of Sciences of the United States of America2009
A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3.Structure (London, England : 1993) · 2023Article
- X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB.Communications biology · 2021Article
- It takes two to tango: The dance of the permease.The Journal of general physiology · 2019Review
- Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.BMC biology · 2018Article
- Thermodynamic cooperativity of cosubstrate binding and cation selectivity ofThe Journal of general physiology · 2017Article
- Structural Insights into the Transport Mechanism of the Human Sodium-dependent Lysophosphatidylcholine Transporter MFSD2A.The Journal of biological chemistry · 2016Article
- Effect of Detergents on Galactoside Binding by Melibiose Permeases.Biochemistry · 2015Article
- The Melibiose Transporter of Escherichia coli: CRITICAL CONTRIBUTION OF LYS-377 TO THE STRUCTURAL ORGANIZATION OF THE INTERACTING SUBSTRATE BINDING SITES.The Journal of biological chemistry · 2015Article
- Thermodynamic mechanism for inhibition of lactose permease by the phosphotransferase protein IIAGlc.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Insights into the inhibitory mechanisms of the regulatory protein IIA(Glc) on melibiose permease activity.The Journal of biological chemistry · 2014Article
- Suppression of conformation-compromised mutants of Salmonella enterica serovar Typhimurium MelB.Journal of bacteriology · 2014Article
- Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H⁺ transporters in fungi and plants.Open biology · 2014Article
- Structure-based mechanism for Na(+)/melibiose symport by MelB.Nature communications · 2014Article
- Two-dimensional crystalline array formation of glucuronide transporter from Escherichia coli by the use of polystyrene beads for detergent removal.The Journal of membrane biology · 2013Article
- Reduced Na+ affinity increases turnover of Salmonella enterica serovar Typhimurium MelB.Journal of bacteriology · 2012Article
- Role of Gly117 in the cation/melibiose symport of MelB of Salmonella typhimurium.Biochemistry · 2012Article
- A haploid genetic screen identifies the major facilitator domain containing 2A (MFSD2A) transporter as a key mediator in the response to tunicamycin.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Mechanism of melibiose/cation symport of the melibiose permease of Salmonella typhimurium.The Journal of biological chemistry · 2011Article
- Structural insights into the activation mechanism of melibiose permease by sodium binding.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Delineating electrogenic reactions during lactose/H+ symport.Biochemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
The melibiose permease of Escherichia coli (MelB) catalyzes the coupled stoichiometric symport of a galactoside with a cation (either Na(+), Li(+), or H(+)), using free energy from the downhill translocation of one cosubstrate to catalyze the accumulation of the other. Here, we present a 3D structure model of MelB threaded through a crystal structure of the lactose permease of E. coli (LacY), manually adjusted, and energetically minimized. The model contains 442 consecutive residues ( approximately 94% of the polypeptide), including all 12 transmembrane helices and connecting loops, with no steric clashes and superimposes well with the template structure. The electrostatic surface potential calculated from the model is typical for a membrane protein and exhibits a characteristic ring of positive charges around the periphery of the cytoplasmic side. The 3D model indicates that MelB consists of two pseudosymmetrical 6-helix bundles lining an internal hydrophilic cavity, which faces the cytoplasmic side of the membrane. Both sugar and cation binding sites are proposed to lie within the internal cavity. The model is consistent with numerous previous mutational, biochemical/biophysical characterizations as well as low-resolution structural data. Thus, an alternating access mechanism with sequential binding is discussed. The proposed overall fold of MelB is different from the available crystal structures of other Na(+)-coupled transporters, suggesting a distinctive fold for Na(+) symporters.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.