ArticleProceedings of the National Academy of Sciences of the United States of America2009
Establishment of trophoblast stem cell lines from somatic cell nuclear-transferred embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Efficient Derivation and Transcriptional Characterization of Mouse Extra-Embryonic Endoderm Stem Cell Lines Generated by Somatic Cell Nuclear Transfer.Stem cells international · 2026Article
- A roadmap towards targeted differentiation of mouse trophoblast stem cells into cell types of the feto-maternal exchange surface.Stem cell research & therapy · 2025Article
- Chimerization of human ESC-derived extraembryonic cells with the mouse blastocyst.International journal of biological sciences · 2024Article
- Early Cell Specification in Mammalian Fertilized and Somatic Cell Nuclear Transfer Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Maintenance of mouse trophoblast stem cells in KSR-based medium allows conventional 3D culture.The Journal of reproduction and development · 2021Article
- Organoids as Novel Models for Embryo Implantation Study.Reproductive sciences (Thousand Oaks, Calif.) · 2021Review
- Differential Transcriptomes and Methylomes of Trophoblast Stem Cells From Naturally-Fertilized and Somatic Cell Nuclear-Transferred Embryos.Frontiers in cell and developmental biology · 2021Article
- Aberrant imprinting in mouse trophoblast stem cells established from somatic cell nuclear transfer-derived embryos.Epigenetics · 2018Article
- Cell Adhesion Minimization by a Novel Mesh Culture Method Mechanically Directs Trophoblast Differentiation and Self-Assembly Organization of Human Pluripotent Stem Cells.Tissue engineering. Part C, Methods · 2015Article
- Derivation and maintenance of murine trophoblast stem cells under defined conditions.Stem cell reports · 2014Article
- Recent advancements in cloning by somatic cell nuclear transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Trophoblast stem cells derived from nuclear transfer embryos: phenotypically unique, bad neighbors, or poor communicators?Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Ontological aspects of pluripotency and stemness gene expression pattern in the rhesus monkey.Gene expression patterns : GEPArticle
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11 authors.
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Abstract
Placental abnormalities occur frequently in cloned animals. Here, we attempted to isolate trophoblast stem (TS) cells from mouse blastocysts produced by somatic cell nuclear transfer (NT) at the blastocyst stage (NT blastocysts). Despite the predicted deficiency of the trophoblast cell lineage, we succeeded in isolating cell colonies with typical morphology of TS cells and cell lines from the NT blastocysts (ntTS cell lines) with efficiency as high as that from native blastocysts. The established 10 ntTS cell lines could be maintained in the undifferentiated state and induced to differentiate into several trophoblast subtypes in vitro. A comprehensive analysis of the transcriptional and epigenetic traits demonstrated that ntTS cells were indistinguishable from control TS cells. In addition, ntTS cells contributed exclusively to the placenta and survived until term in chimeras, indicating that ntTS cells have developmental potential as stem cells. Taken together, our data show that NT blastocysts contain cells that can produce TS cells in culture, suggesting that proper commitment to the trophoblast cell lineage in NT embryos occurs by the blastocyst stage.
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