ArticleCell proliferation2009
Differential effects of Nucleostemin suppression on cell cycle arrest and apoptosis in the bladder cancer cell lines 5637 and SW1710.
Article in Cell proliferation, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Association of Nucleostemin Polymorphisms with Chronic Hepatitis B Virus Infection in Chinese Han Population.Genetic testing and molecular biomarkers · 2022Article
- Silencing of Nucleostemin by siRNA Induces Apoptosis in MCF-7 and MDA-MB-468 Cell Lines.Iranian journal of pharmaceutical research : IJPR · 2020Article
- Differential expression of nucleostemin in the cytoplasm and nuclei of normal and cancerous cell lines.Turkish journal of biology = Turk biyoloji dergisi · 2018Article
- Gene expression profiling of NB4 cells following knockdown of nucleostemin using DNA microarrays.Molecular medicine reports · 2016Article
- Balancing self-renewal against genome preservation in stem cells: How do they manage to have the cake and eat it too?Cellular and molecular life sciences : CMLS · 2016Review
- Nucleostemin Knockdown Sensitizes Hepatocellular Carcinoma Cells to Ultraviolet and Serum Starvation-Induced Apoptosis.PloS one · 2015Article
- Guanine nucleotide binding protein-like 3 is a potential prognosis indicator of gastric cancer.International journal of clinical and experimental pathology · 2015Article
- Expression profile of ZFX isoform3/variant 5 in gastric cancer tissues and its association with tumor size.Iranian journal of basic medical sciences · 2014Article
- Turning a new page on nucleostemin and self-renewal.Journal of cell science · 2014Article
- Concomitant upregulation of nucleostemin and downregulation of Sox2 and Klf4 in gastric adenocarcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- Nucleostemin and GNL3L exercise distinct functions in genome protection and ribosome synthesis, respectively.Journal of cell science · 2014Article
- EYA1 expression in gastric carcinoma and its association with clinicopathological characteristics: a pilot study.Medical oncology (Northwood, London, England) · 2014Article
- Nucleostemin depletion induces post-g1 arrest apoptosis in chronic myelogenous leukemia k562 cells.Advanced pharmaceutical bulletin · 2014Article
- Immunohistochemical study of doublecortin and nucleostemin in canine brain.European journal of histochemistry : EJH · 2013Article
- Identification of specific pluripotent stem cell death--inducing small molecules by chemical screening.Stem cell reviews and reports · 2012Article
- Differential expression of ZFX gene in gastric cancer.Journal of biosciences · 2012Article
- Article
- Reactive oxygen species regulate nucleostemin oligomerization and protein degradation.The Journal of biological chemistry · 2011Article
- An off-target nucleostemin RNAi inhibits growth in human glioblastoma-derived cancer stem cells.PloS one · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe Nucleostemin (NS) gene encodes a nucleolar protein enriched in adult and embryonic stem cells. NS is thought to regulate cancer cell proliferation, but the mechanisms involved are poorly understood. In this study, we have investigated the role of NS in bladder cancer. MATERIALS AND
methodsExpression of NS was determined by quantitative reverse transcription-polymerase chain reaction in bladder carcinoma cell lines and in normal uro-epithelial cell cultures. We used an RNAi strategy to investigate the function of NS in two selected carcinoma cell lines.
resultsHigh NS expression was found in most bladder carcinoma cell lines and normal uro-epithelial cells. Knockdown of NS expression induced a severe decline in cell proliferation in 5637 and SW1710 cell lines, both with mutant p53. Apoptosis was more strongly enhanced in 5637 cells lacking RB1 than in SW1710 cells lacking p16(INK4A). Moreover, NS-siRNA-treated 5637 cells accumulated mainly in G(2)/M, whereas SW1710 cells arrested in G(0)/G(1).
conclusionOur data indicate that NS expression is necessary for cell proliferation and evasion of apoptosis in bladder cancer cells, independent of its effect on p53. Also, we speculate that the precise effect of NS on cell cycle regulation may relate to functional status of RB1 and CDKN2A/p16(INK4A).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.