ArticleCommunicative & integrative biology2009
On the role of intravesicular calcium in the motion and exocytosis of secretory organelles.
Article in Communicative & integrative biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Chromaffin Versus Platelet Granules: What We Have Learned From Chromaffin Cells for Human Studies.Journal of neurochemistry · 2026Review
- Article
- Localization and Absolute Quantification of Dopamine in Discrete Intravesicular Compartments Using NanoSIMS Imaging.International journal of molecular sciences · 2021Article
- Generation of interconnected vesicles in a liposomal cell model.Scientific reports · 2020Article
- Readily Releasable Stores of Calcium in Neuronal Endolysosomes: Physiological and Pathophysiological Relevance.Advances in experimental medicine and biology · 2020Review
- Involvement of organelles and inter-organellar signaling in the pathogenesis of HIV-1 associated neurocognitive disorder and Alzheimer's disease.Brain research · 2019Review
- Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Amperometry methods for monitoring vesicular quantal size and regulation of exocytosis release.Pflugers Archiv : European journal of physiology · 2018Review
- Adrenal Chromaffin Cells Exposed to 5-ns Pulses Require Higher Electric Fields to Porate Intracellular Membranes than the Plasma Membrane: An Experimental and Modeling Study.The Journal of membrane biology · 2017Article
- Release of calcium from endolysosomes increases calcium influx through N-type calcium channels: Evidence for acidic store-operated calcium entry in neurons.Cell calcium · 2015Article
- Synaptic vesicles control the time course of neurotransmitter secretion via a Ca²+/H+ antiport.The Journal of physiology · 2011Article
- Intravesicular factors controlling exocytosis in chromaffin cells.Cellular and molecular neurobiology · 2010Article
- Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory vesicles of sympathetic neurons and chromaffin granules maintain a pH gradient towards the cytosol (5.5 vs. 7.2) promoted by the V-ATPase activity. This gradient of pH is mainly responsible for the accumulation of amines. The secretory vesicles contain large amounts of total Ca(2+), but the free intragranular [Ca(2+)], the mechanisms for Ca(2+) uptake and release from the granules and their physiological relevance regarding exocytosis are still matters of debate.We have recently shown that disruption of the pH gradient of secretory vesicles slowed down exocytosis. Fluorimetric measurements, using the dye Oregon green BAPTA-2, showed that the V-ATPase inhibitor bafilomycin A1 directly released Ca(2+) from freshly isolated vesicles. Accordingly, vesicle alkalinization released Ca(2+) from the granules to the cytosol, measured with fura-2 in intact chromaffin cells. Using TIRFM in cells overexpressing the EGFP-labeled synaptobrevin (VAMP2-EGFP) protein, we have then shown that the Ca(2+) released from the vesicles to the cytosol in the presence of bafilomycin, dramatically increased the granule motion of chromaffin- or PC12-derived granules, and triggered exocytosis (measured by amperometry).We conclude that the gradient of pH of secretory vesicles might be involved in the homeostatic regulation of the local cytosolic Ca(2+) around the vesicles and in two of the major functions of secretory cells, vesicle motion and exocytosis.1.
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