ArticlePlant signaling & behavior2007
Membrane contact sites: physical attachment between chloroplasts and endoplasmic reticulum revealed by optical manipulation.
Article in Plant signaling & behavior, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- RH3 enhances antiviral defense by facilitating small RNA loading into Argonaute 2 at endoplasmic reticulum-chloroplast membrane contact sites.Nature communications · 2025Article
- Recovery of the cortical chloroplast layer in the green algaFrontiers in plant science · 2025Article
- SIMPEL: using stable isotopes to elucidate dynamics of context specific metabolism.Communications biology · 2024Article
- Lipid peroxidation and stress-induced signalling molecules in systemic resistance mediated by azelaic acid/AZELAIC ACID INDUCED1: signal initiation and propagation.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Is a spice missing from the recipe? The intra-cellular localization of vanillin biosynthesis needs further investigations.Plant biology (Stuttgart, Germany) · 2023Article
- Friend or foe: Hybrid proline-rich proteins determine how plants respond to beneficial and pathogenic microbes.Plant physiology · 2022Article
- The Passage of HPlants (Basel, Switzerland) · 2022Review
- Sunflower resistance to multiple downy mildew pathotypes revealed by recognition of conserved effectors of the oomycete Plasmopara halstedii.The Plant journal : for cell and molecular biology · 2019Article
- The Arabidopsis COPII components, AtSEC23A and AtSEC23D, are essential for pollen wall development and exine patterning.Journal of experimental botany · 2018Article
- Plastid-produced interorgannellar stress signal MEcPP potentiates induction of the unfolded protein response in endoplasmic reticulum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Chloroplast signaling within, between and beyond cells.Frontiers in plant science · 2015Review
- Membrane glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum.Plant physiology · 2015Article
- ER - the key to the highway.Current opinion in plant biology · 2014Article
- Bridging the gap: membrane contact sites in signaling, metabolism, and organelle dynamics.The Journal of cell biology · 2014Article
- Conditional Depletion of the Chlamydomonas Chloroplast ClpP Protease Activates Nuclear Genes Involved in Autophagy and Plastid Protein Quality Control.The Plant cell · 2014Article
- Unknown components of the plastidial permeome.Frontiers in plant science · 2014Review
- How protein targeting to primary plastids via the endomembrane system could have evolved? A new hypothesis based on phylogenetic studies.Biology direct · 2013Article
- New technologies for 21st century plant science.The Plant cell · 2012Article
- Plastid stromule branching coincides with contiguous endoplasmic reticulum dynamics.Plant physiology · 2011Article
- The Plastid Outer Envelope - A Highly Dynamic Interface between Plastid and Cytoplasm.Frontiers in plant science · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts and their surrounding cell are highly interdependent. One example is lipid metabolism, where the cell depends on its chloroplasts to provide fatty acids for lipid synthesis in the endoplasmic reticulum (ER) and in turn, chloroplasts rely on import of lipid precursors from the ER. Despite its fundamental importance, the route for lipid trafficking into and out of chloroplasts remains unknown. Biochemical studies of plant membrane lipid metabolism have suggested the possibility of lipid transport at membrane contact sites (MCSs) between the ER and chloroplasts. With the aid of optical manipulation, we recently could present physical evidence for this association. Leaf protoplasts isolated from Arabidopsis thaliana expressing green fluorescent protein (GFP) in the ER lumen were observed by confocal microscopy. A laser scalpel was used to rupture the protoplasts. ER fragments associated with the released chloroplasts could be stretched out by optical tweezers but remained attached to the chloroplast surface, even when a stretching force of 400 pN was applied. We thus provided the first physical evidence for MCSs between two membranes and we propose for the ER-chloroplast pair, that such tight associations are involved in bidirectional lipid trafficking between the two compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.