ArticleGenetics2009
Adaptive divergence in experimental populations of Pseudomonas fluorescens. IV. Genetic constraints guide evolutionary trajectories in a parallel adaptive radiation.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- TheVirulence · 2026Article
- Genetic diversification ofApplied and environmental microbiology · 2026Article
- Thermoregulation of functional amyloid Fap-dependent biofilm formation via cyclic diguanosine monophosphate signaling inApplied and environmental microbiology · 2026Article
- Historical contingency limits adaptive diversification in a spatially structured environment.Evolution letters · 2026Article
- Cyclic-di-GMP signalling mutants drive ecological succession and self-generated diversity in experimentally evolved biofilms ofMicrobiology (Reading, England) · 2025Article
- Student-led experimental evolution reveals novel biofilm regulators of adaptation to multiple niches.bioRxiv : the preprint server for biology · 2025Article
- Evolution of genotypic and phenotypic diversity in multispecies biofilms.NPJ biofilms and microbiomes · 2025Article
- Co-Existence Slows Diversification in Experimental Populations of E. coli and P. fluorescens.Environmental microbiology · 2025Article
- Hypermutability bypasses genetic constraints in SCV phenotypic switching in Pseudomonas aeruginosa biofilms.NPJ biofilms and microbiomes · 2025Article
- Extending evolutionary forecasts across bacterial species.Proceedings. Biological sciences · 2024Article
- Genomic sequencing reveals convergent adaptation during experimental evolution in two budding yeast species.Communications biology · 2024Article
- Polar accumulation of pyoverdin and exit from stationary phase.microLife · 2024Article
- Phenotype Design Space Provides a Mechanistic Framework Relating Molecular Parameters to Phenotype Diversity Available for Selection.Journal of molecular evolution · 2023Article
- Identifying Targets of Selection in Laboratory Evolution Experiments.Journal of molecular evolution · 2023Review
- Evolution of cyclic di-GMP signalling on a short and long term time scale.Microbiology (Reading, England) · 2023Article
- Distribution of mutation rates challenges evolutionary predictability.Microbiology (Reading, England) · 2023Article
- Article
- Evolutionary Trajectories are Contingent on Mitonuclear Interactions.Molecular biology and evolution · 2023Article
- Article
- Towards evolutionary predictions: Current promises and challenges.Evolutionary applications · 2023Review
22 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
The capacity for phenotypic evolution is dependent upon complex webs of functional interactions that connect genotype and phenotype. Wrinkly spreader (WS) genotypes arise repeatedly during the course of a model Pseudomonas adaptive radiation. Previous work showed that the evolution of WS variation was explained in part by spontaneous mutations in wspF, a component of the Wsp-signaling module, but also drew attention to the existence of unknown mutational causes. Here, we identify two new mutational pathways (Aws and Mws) that allow realization of the WS phenotype: in common with the Wsp module these pathways contain a di-guanylate cyclase-encoding gene subject to negative regulation. Together, mutations in the Wsp, Aws, and Mws regulatory modules account for the spectrum of WS phenotype-generating mutations found among a collection of 26 spontaneously arising WS genotypes obtained from independent adaptive radiations. Despite a large number of potential mutational pathways, the repeated discovery of mutations in a small number of loci (parallel evolution) prompted the construction of an ancestral genotype devoid of known (Wsp, Aws, and Mws) regulatory modules to see whether the types derived from this genotype could converge upon the WS phenotype via a novel route. Such types-with equivalent fitness effects-did emerge, although they took significantly longer to do so. Together our data provide an explanation for why WS evolution follows a limited number of mutational pathways and show how genetic architecture can bias the molecular variation presented to selection.
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