ArticleInfection and immunity2009
Nondividing but metabolically active gamma-irradiated Brucella melitensis is protective against virulent B. melitensis challenge in mice.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Gamma Irradiated Pasteurella multocida Vaccine induces strong humoral immunity and protects rabbits from disease.Veterinary research communications · 2024Article
- Synthetic control of living cells by intracellular polymerization.Trends in biotechnology · 2024Review
- Improved Whole Gamma Irradiated Avian Influenza Subtype H9N2 Virus Vaccine Using Trehalose and Optimization of Vaccination Regime on Broiler Chicken.Frontiers in veterinary science · 2022Article
- Advances in Irradiated Livestock Vaccine Research and Production Addressing the Unmet Needs for Farmers and Veterinary Services in FAO/IAEA Member States.Frontiers in immunology · 2022Review
- Protection against virulentClinical and experimental vaccine research · 2022Article
- Irradiated Non-replicative Lactic Acid Bacteria Preserve Metabolic Activity While Exhibiting Diverse Immune Modulation.Frontiers in veterinary science · 2022Article
- Ionizing Radiation Technologies for Vaccine Development - A Mini Review.Frontiers in immunology · 2022Review
- Article
- Controlling the Colonization ofAnimals : an open access journal from MDPI · 2021Article
- Characterization of humoral and cellular immune features of gamma-irradiated influenza vaccine.Human vaccines & immunotherapeutics · 2021Article
- Article
- IL-6 and INF-γ levels in patients with brucellosis in severe epidemic region, Xinjiang, China.Infectious diseases of poverty · 2020Article
- Proteomic Analysis of Vesicle-ProducingFrontiers in microbiology · 2020Article
- Article
- Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis.Scientific reports · 2017Article
- The Case for Live Attenuated Vaccines against the Neglected Zoonotic Diseases Brucellosis and Bovine Tuberculosis.PLoS neglected tropical diseases · 2016Review
- X-ray Irradiated Vaccine Confers protection against Pneumonia caused by Pseudomonas aeruginosa.Scientific reports · 2016Article
- Application of radiation technology in vaccines development.Clinical and experimental vaccine research · 2015Review
- Virulent Francisella tularensis destabilize host mRNA to rapidly suppress inflammation.Journal of innate immunity · 2014Article
- Studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging.Methods in molecular biology (Clifton, N.J.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Brucella spp. are gram-negative bacteria that cause the most frequent zoonotic disease worldwide, with more than 500,000 human infections yearly; however, no human vaccine is currently available. As with other intracellular organisms, cytotoxic mechanisms against infected cells are thought to have an important role in controlling infection and mediating long-term immunity. Live attenuated strains developed for use in animals elicit protection but retain unacceptable levels of virulence. Thus, the optimal design for a brucellosis vaccine requires a nonliving vaccine that confers effective immunity. Historically, inactivation methods such as chemical or heat treatment successfully impair Brucella reproductive capacity; nevertheless, metabolically inactive vaccines (subunit or killed) present very limited efficacy. Hence, we hypothesized that bacterial metabolism plays a major role in creating the proper antigenic and adjuvant properties required for efficient triggering of protective responses. Here, we demonstrate that inactivation of Brucella melitensis by gamma-irradiation inhibited its replication capability and yet retained live-Brucella protective features. Irradiated Brucella possessed metabolic and transcriptional activity, persisted in macrophages, generated antigen-specific cytotoxic T cells, and protected mice against virulent bacterial challenge, without signs of residual virulence. In conclusion, pathogen metabolic activity has a positive role in shaping protective responses, and the generation of inactivated and yet metabolically active microbes is a promising strategy for safely vaccinating against intracellular organisms such as B. melitensis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.