ArticleThe Journal of physiology2009
A Ca(2+)-activated Cl(-) conductance in interstitial cells of Cajal linked to slow wave currents and pacemaker activity.
Article in The Journal of physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 178 papers, 1 of them a synthesis that pooled it.
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Who cites it
178 citing papers in PubMed, 1 synthesis or guideline pooled it, 264 citations in OpenAlex.
- Electrogastrography measurement systems and analysis methods used in clinical practice and research: comprehensive review.Frontiers in medicine · 2024Pooled it
- Cells and ionic conductances contributing to spontaneous activity in bladder and urethral smooth muscle.The Journal of physiology · 2026Review
- Pacemaking in the lymphatic system.The Journal of physiology · 2026Review
- Conformational changes upon pore blocker removal reveal conductive states of TMEM16A.bioRxiv : the preprint server for biology · 2026Article
- Role of ANO1 CaPflugers Archiv : European journal of physiology · 2025Article
- ER morphological analysis associated with interstitial cells of Cajal and smooth muscle cells in the murine stomach.Cell and tissue research · 2025Article
- Neuronal regulation of myenteric interstitial cells of Cajal (ICC-MY) in the proximal colon.Cell calcium · 2025Article
- TMEM16A in gastrointestinal and vascular smooth muscle contractility.The Journal of physiology · 2025Review
- Article
- ANO1 channels are expressed in mouse urethral smooth muscle but do not contribute to agonist or neurally evoked contractions.Scientific reports · 2025Article
- A specialized population of intramuscular interstitial cells of Cajal regulates motility within the mouse oesophagogastric junction.The Journal of physiology · 2025Article
- Attenuation of Ampullary Anoctamin 1 by the peritoneal fluid in rhesus macaques with spontaneous endometriosis†.Biology of reproduction · 2025Article
- Immunohistochemical characterization of interstitial cells and their spatial relationship to motor neurons within the mouse esophagus.Cell and tissue research · 2025Article
- Article
- Modulation of intracellular calcium activity in interstitial cells of Cajal by inhibitory neural pathways within the internal anal sphincter.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Article
- Immunohistochemical characterization of interstitial cells and their relationship to motor neurons within the mouse esophagus.Research square · 2024Article
- A bibliometric analysis of interstitial cells of Cajal research.Frontiers in medicine · 2024Review
- Function and Regulation of the Calcium-Activated Chloride Channel Anoctamin 1 (TMEM16A).Handbook of experimental pharmacology · 2024Article
- CaPhysiological reviews · 2024Review
118 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Interstitial cells of Cajal (ICC) are unique cells that generate electrical pacemaker activity in gastrointestinal (GI) muscles. Many previous studies have attempted to characterize the conductances responsible for pacemaker current and slow waves in the GI tract, but the precise mechanism of electrical rhythmicity is still debated. We used a new transgenic mouse with a bright green fluorescent protein (copGFP) constitutively expressed in ICC to facilitate study of these cells in mixed cell dispersions. We found that ICC express a specialized 'slow wave' current. Reversal of tail current analysis showed this current was due to a Cl(-) selective conductance. ICC express ANO1, a Ca(2+)-activated Cl(-) channel. Slow wave currents are not voltage dependent, but a secondary voltage-dependent process underlies activation of these currents. Removal of extracellular Ca(2+), replacement of Ca(2+) with Ba(2+), or extracellular Ni(2+) (30 microm) blocked the slow wave current. Single Ca(2+)-activated Cl() channels with a unitary conductance of 7.8 pS were resolved in excised patches of ICC. These are similar in conductance to ANO1 channels (8 pS) expressed in HEK293 cells. Slow wave current was blocked in a concentration-dependent manner by niflumic acid (IC(50) = 4.8 microm). Slow wave currents are associated with transient depolarizations of ICC in current clamp, and these events were blocked by niflumic acid. These findings demonstrate a role for a Ca(2+)-activated Cl(-) conductance in slow wave current in ICC and are consistent with the idea that ANO1 participates in pacemaker activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.