ArticleThe Journal of biological chemistry2009
Cyclin A/cdk2 regulates adenomatous polyposis coli-dependent mitotic spindle anchoring.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- Cyclin A-CDK1 suppresses the expression of the CDK1 activator CDC25A to safeguard timely mitotic entry.The Journal of biological chemistry · 2023Article
- Role of Stress-Survival Pathways and Transcriptomic Alterations in Progression of Colorectal Cancer: A Health Disparities Perspective.International journal of environmental research and public health · 2021Review
- Development of Human Monoclonal Antibody for Claudin-3 Overexpressing Carcinoma Targeting.Biomolecules · 2019Article
- Wnt Signaling and Its Impact on Mitochondrial and Cell Cycle Dynamics in Pluripotent Stem Cells.Genes · 2018Review
- Tumor suppressor APC is an attenuator of spindle-pulling forces duringProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Lupeol inhibits growth and migration in two human colorectal cancer cell lines by suppression of Wnt-β-catenin pathway.OncoTargets and therapy · 2018Article
- Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation.Science (New York, N.Y.) · 2016Article
- MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C.Biology open · 2015Article
- Cyclin A/Cdk2 regulates Cdh1 and claspin during late S/G2 phase of the cell cycle.Cell cycle (Georgetown, Tex.) · 2014Article
- Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balance.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Cyclin A2, Rho GTPases and EMT.Small GTPasesArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutations in adenomatous polyposis coli (APC) protein is a major contributor to tumor initiation and progression in several tumor types. These mutations affect APC function in the Wnt-beta-catenin signaling and influence mitotic spindle anchoring to the cell cortex and orientation. Here we report that the mitotic anchoring and orientation function of APC is regulated by cyclin A/cdk2. Knockdown of cyclin A and inhibition of cdk2 resulted in cells arrested in mitosis with activation of the spindle assembly checkpoint. The mitotic spindle was unable to form stable attachments to the cell cortex, and this resulted in the spindles failing to locate to the central position in the cells and undergo dramatic rotation. We have demonstrated that cyclin A/cdk2 specifically associates with APC in late G2 phase and phosphorylates it at Ser-1360, located in the mutation cluster region of APC. Mutation of APC Ser-1360 to Ala results in identical off-centered mitotic spindles. Thus, this cyclin A/cdk2-dependent phosphorylation of APC affects astral microtubule attachment to the cortical surface in mitosis.
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Registered trials
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