ArticleCell2009
Transposition into replicating DNA occurs through interaction with the processivity factor.
Article in Cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Phage Mu enlists the β-sliding clamp for late gene transcription.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Asymmetric loading of TnsE regulates Tn7 targeting of DNA replication structures.Nucleic acids research · 2025Article
- A family of Tn7-like transposons evolved to target CRISPR repeats.Mobile DNA · 2025Article
- Article
- Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor.Nucleic acids research · 2024Article
- Natural and Engineered Guide RNA-Directed Transposition with CRISPR-Associated Tn7-Like Transposons.Annual review of biochemistry · 2024Review
- DNA on the move: mechanisms, functions and applications of transposable elements.FEBS open bio · 2024Review
- Transposons and CRISPR: Rewiring Gene Editing.Biochemistry · 2023Article
- Precise cut-and-paste DNA insertion using engineered type V-K CRISPR-associated transposases.Nature biotechnology · 2023Article
- Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition.Molecular cell · 2023Article
- Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria.Nucleic acids research · 2023Article
- Review
- Cargo Genes of TnmBio · 2021Article
- Article
- Article
- Targeted transposition with Tn7 elements: safe sites, mobile plasmids, CRISPR/Cas and beyond.Molecular microbiology · 2019Review
- A group II intron-encoded protein interacts with the cellular replicative machinery through the β-sliding clamp.Nucleic acids research · 2019Article
- CRISPR-Cas in mobile genetic elements: counter-defence and beyond.Nature reviews. Microbiology · 2019Article
- Article
- Single-strand DNA processing: phylogenomics and sequence diversity of a superfamily of potential prokaryotic HuH endonucleases.BMC genomics · 2018Article
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6 authors.
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Abstract
The bacterial transposon Tn7 directs transposition into actively replicating DNA by a mechanism involving the transposon-encoded protein TnsE. Here we show that TnsE physically and functionally interacts with the processivity factor of the DNA replication machinery in vivo and in vitro. Our work establishes an in vitro TnsABC+E transposition reaction reconstituted from purified proteins and target DNA structures. Using the in vitro reaction we confirm that the processivity factor specifically reorders TnsE-mediated transposition events on target DNAs in a way that matches the bias with active DNA replication in vivo. The TnsE interaction with an essential and conserved component of the replication machinery, and a DNA structure reveals a mechanism by which Tn7, and probably other elements, selects target sites associated with DNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.