ArticleCellular microbiology2009
Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes.
Article in Cellular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- The atypical dual-specificity protein phosphatase (DUSP)/kinatase of Leishmania infantum modulates infectivity, oxidative stress response and antimonial resistance.PLoS neglected tropical diseases · 2026Article
- Unrevealing the Mystery of Latent Leishmaniasis: What Cells Can HostPathogens (Basel, Switzerland) · 2023Review
- Article
- Epidemiologic, Clinical and Immunological Consequences of Co-Infections during Canine Leishmaniosis.Animals : an open access journal from MDPI · 2021Review
- Complement receptor 3 mediates ruffle-like, actin-rich aggregates during phagocytosis of Leishmania infantum metacyclics.Experimental parasitology · 2021Article
- The macrophage microtubule network acts as a key cellular controller of the intracellular fate of Leishmania infantum.PLoS neglected tropical diseases · 2020Article
- Sex-Related Differences in Immune Response and Symptomatic Manifestations to Infection withJournal of immunology research · 2019Review
- Deciphering the Role Played by Autophagy inFrontiers in immunology · 2019Review
- The Phenotype of Circulating Neutrophils during Visceral Leishmaniasis.The American journal of tropical medicine and hygiene · 2017Article
- The site of the bite: Leishmania interaction with macrophages, neutrophils and the extracellular matrix in the dermis.Parasites & vectors · 2016Review
- Innate immunity against Leishmania infections.Cellular microbiology · 2015Review
- In vitro metacyclogenesis of Leishmania (Viannia) braziliensis and Leishmania (Leishmania) amazonensis clinical field isolates, as evaluated by morphology, complement resistance, and infectivity to human macrophages.BioMed research international · 2015Article
- Immunoregulation in human American leishmaniasis: balancing pathology and protection.Parasite immunology · 2014Review
- Deception and manipulation: the arms of leishmania, a successful parasite.Frontiers in immunology · 2014Review
- Attenuation of Leishmania infantum chagasi metacyclic promastigotes by sterol depletion.Infection and immunity · 2013Article
- Transmission and epidemiology of zoonotic protozoal diseases of companion animals.Clinical microbiology reviews · 2013Review
- Sink or swim: lipid rafts in parasite pathogenesis.Trends in parasitology · 2012Review
- Receptor-mediated phagocytosis of Leishmania: implications for intracellular survival.Trends in parasitology · 2012Review
- Recent developments in the interactions between caveolin and pathogens.Advances in experimental medicine and biology · 2012Review
- The effects of macrophage source on the mechanism of phagocytosis and intracellular survival of Leishmania.Microbes and infection · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The obligate intracellular protozoan, Leishmania infantum chagasi (Lic) undergoes receptor-mediated phagocytosis by macrophages followed by a transient delay in phagolysosome maturation. We found differences in the pathway through which virulent Lic metacyclic promastigotes or avirulent logarithmic promastigotes are phagocytosed by human monocyte-derived macrophages (MDMs). Both logarithmic and metacyclic promastigotes entered MDMs through a compartment lined by the third complement receptor (CR3). In contrast, many logarithmic promastigotes entered through vacuoles lined by mannose receptors (MR) whereas most metacyclic promastigotes did not (P < 0.005). CR3-positive vacuoles containing metacyclic promastigotes stained for caveolin-1 protein, suggesting CR3 localizes in caveolae during phagocytosis. Following entry, the kinetics of phagolysosomal maturation and intracellular survival also differed. Vacuoles containing metacyclic parasites did not accumulate lysosome-associated membrane protein-1 (LAMP-1) at early times after phagocytosis, whereas vacuoles with logarithmic promastigotes did. MDMs phagocytosed greater numbers of logarithmic than metacyclic promastigotes, yet metacyclics ultimately replicated intracellularly with greater efficiency. These data suggest that virulent metacyclic Leishmania promastigotes fail to ligate macrophage MR, and enter through a path that ultimately enhances intracellular survival. The relatively quiescent entry of virulent Leishmania spp. into macrophages may be accounted for by the ability of metacyclic promastigotes to selectively bypass deleterious entry pathways.
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Registered trials
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