ArticleNature structural & molecular biology2009
Fast ribozyme cleavage releases transcripts from RNA polymerase II and aborts co-transcriptional pre-mRNA processing.
Article in Nature structural & molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Emerging and re-emerging themes in co-transcriptional pre-mRNA splicing.Molecular cell · 2024Review
- DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3'-end of genes and involved in the recycling of cleavage factors.Nature communications · 2024Article
- Inhibition ofeLife · 2024Article
- A mechanistic view of long noncoding RNAs in cancer.Wiley interdisciplinary reviews. RNA · 2022Review
- 5'-Cap sequestration is an essential determinant of HIV-1 genome packaging.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Herpes simplex virus blocks host transcription termination via the bimodal activities of ICP27.Nature communications · 2020Article
- A unified allosteric/torpedo mechanism for transcriptional termination on human protein-coding genes.Genes & development · 2020Article
- R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters.Molecular cell · 2017Article
- Lariat capping as a tool to manipulate the 5' end of individual yeast mRNA species in vivo.RNA (New York, N.Y.) · 2017Article
- Chemistry and Biology of Self-Cleaving Ribozymes.Trends in biochemical sciences · 2015Review
- Poly(A) Polymerase and the Nuclear Poly(A) Binding Protein, PABPN1, Coordinate the Splicing and Degradation of a Subset of Human Pre-mRNAs.Molecular and cellular biology · 2015Article
- Co-transcriptional mRNP formation is coordinated within a molecular mRNP packaging station in S. cerevisiae.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- mRNA quality control goes transcriptional.Biochemical Society transactions · 2013Review
- Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II.PLoS genetics · 2013Article
- Biological significance of RNA editing in cells.Molecular biotechnology · 2012Review
- An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.Molecular and cellular biology · 2012Article
- mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription.Molecular cell · 2012Article
- Pri-miR-17-92a transcript folds into a tertiary structure and autoregulates its processing.RNA (New York, N.Y.) · 2012Article
- DBIRD complex integrates alternative mRNA splicing with RNA polymerase II transcript elongation.Nature · 2012Article
- Pre-mRNA splicing is a determinant of histone H3K36 methylation.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
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3 authors.
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Abstract
We investigated whether a continuous transcript is necessary for co-transcriptional pre-mRNA processing. Cutting an intron with the fast-cleaving hepatitis delta ribozyme, but not the slower hammerhead, inhibited splicing. Therefore, exon tethering to RNA polymerase II (Pol II) cannot rescue splicing of a transcript severed by a ribozyme that cleaves rapidly relative to the rate of splicing. Ribozyme cutting also released cap-binding complex (CBC) from the gene, suggesting that exon 1 is not tethered. Unexpectedly, cutting within exons inhibited splicing of distal introns, where exon definition is not affected, probably owing to disruption of the interactions with the CBC and the Pol II C-terminal domain that facilitate splicing. Ribozyme cutting within the mRNA also inhibited 3' processing and transcription termination. We propose that damaging the nascent transcript aborts pre-mRNA processing and that this mechanism may help to prevent association of processing factors with Pol II that is not productively engaged in transcription.
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