ArticleNucleic acids research2009
An N-terminal clamp restrains the motor domains of the bacterial transcription-repair coupling factor Mfd.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Structural basis for loading of transcription repair-coupling factor Mfd onto stalled elongation complexes.Nucleic acids research · 2026Article
- Structural basis for loading of Transcription Repair-Coupling factor Mfd onto stalled elongation complexes.bioRxiv : the preprint server for biology · 2025Article
- Single-molecule studies of helicases and translocases in prokaryotic genome-maintenance pathways.DNA repair · 2021Review
- Mfd - at the crossroads of bacterial DNA repair, transcriptional regulation and molecular evolvability.Transcription · 2021Review
- Article
- A Peek Inside the Machines of Bacterial Nucleotide Excision Repair.International journal of molecular sciences · 2021Review
- Within and Beyond the Nucleotide Addition Cycle of Viral RNA-dependent RNA Polymerases.Frontiers in molecular biosciences · 2021Article
- Structural basis of Mfd-dependent transcription termination.Nucleic acids research · 2020Article
- Molecular determinants for dsDNA translocation by the transcription-repair coupling and evolvability factor Mfd.Nature communications · 2020Article
- Single-molecule imaging reveals molecular coupling between transcription and DNA repair machinery in live cells.Nature communications · 2020Article
- Uncoupling of ProteaseJournal of virology · 2017Article
- Mfd Protein and Transcription-Repair Coupling in Escherichia coli.Photochemistry and photobiology · 2017Review
- A tale of two cities: A tribute to Aziz Sancar's Nobel Prize in Chemistry for his molecular characterization of NER.DNA repair · 2016Article
- A dynamic DNA-repair complex observed by correlative single-molecule nanomanipulation and fluorescence.Nature structural & molecular biology · 2015Article
- Regulation of Transcript Elongation.Annual review of microbiology · 2015Review
- UvrD helicase: an old dog with a new trick: how one step backward leads to many steps forward.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Regulation of the Rhp26ERCC6/CSB chromatin remodeler by a novel conserved leucine latch motif.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- RNA polymerase between lesion bypass and DNA repair.Cellular and molecular life sciences : CMLS · 2013Review
- Prokaryotic nucleotide excision repair.Cold Spring Harbor perspectives in biology · 2013Review
- Interplay of DNA repair with transcription: from structures to mechanisms.Trends in biochemical sciences · 2012Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Motor proteins that translocate on nucleic acids are key players in gene expression and maintenance. While the function of these proteins is diverse, they are driven by highly conserved core motor domains. In transcription-coupled DNA repair, motor activity serves to remove RNA polymerase stalled on damaged DNA, making the lesion accessible for repair. Structural and biochemical data on the bacterial transcription-repair coupling factor Mfd suggest that this enzyme undergoes large conformational changes from a dormant state to an active state upon substrate binding. Mfd can be functionally dissected into an N-terminal part instrumental in recruiting DNA repair proteins (domains 1-3, MfdN), and a C-terminal part harboring motor activity (domains 4-7, MfdC). We show that isolated MfdC has elevated ATPase and motor activities compared to the full length protein. While MfdN has large effects on MfdC activity and thermostability in cis, these effects are not observed in trans. The structure of MfdN is independent of interactions with MfdC, implying that MfdN acts as a clamp that restrains motions of the motor domains in the dormant state. We conclude that releasing MfdN:MfdC interactions serves as a central molecular switch that upregulates Mfd functions during transcription-coupled DNA repair.
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