ArticleThe Plant cell2009
Control of nuclear and nucleolar localization of nuclear inclusion protein a of picorna-like Potato virus A in Nicotiana species.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 156 citations in OpenAlex.
- Antiviral RNA interference in plants: Increasing complexity and integration with other biological processes.Plant communications · 2025Review
- Nucleolar fibrillarin methyltransferase regulates systemic trafficking of a plant virus satellite RNA.The Plant cell · 2025Article
- Natural self-attenuation of pathogenic viruses by deleting the silencing suppressor coding sequence for long-term plant-virus coexistence.PLoS pathogens · 2025Article
- Turnip mosaic virus infection cleaves MEDIATOR SUBUNIT16 in plants increasing plant susceptibility to the virus and its aphid vector Myzus persicae.BMC plant biology · 2025Article
- Biomolecular condensates in plant RNA silencing: insights into formation, function, and stress responses.The Plant cell · 2024Review
- Advances in the structure and function of the nucleolar protein fibrillarin.Frontiers in cell and developmental biology · 2024Review
- The overlooked manipulation of nucleolar functions by plant pathogen effectors.Frontiers in plant science · 2024Review
- Identification and characterization of theFrontiers in plant science · 2024Article
- The mystery remains: How do potyviruses move within and between cells?Molecular plant pathology · 2023Review
- Review
- The Phytophthora nucleolar effector Pi23226 targets host ribosome biogenesis to induce necrotrophic cell death.Plant communications · 2023Article
- Cajal bodies: Evolutionarily conserved nuclear biomolecular condensates with properties unique to plants.The Plant cell · 2023Review
- Rapid selection of potyviral cross-protection effective mutants from the local lesion host after nitrous acid mutagenesis.Molecular plant pathology · 2023Article
- Candidate genes for field resistance to cassava brown streak disease revealed through the analysis of multiple data sources.Frontiers in plant science · 2023Article
- Ryegrass mottle virus complete genome determination and development of infectious cDNA by combining two methods- 3' RACE and RNA-Seq.PloS one · 2023Article
- A Viral Suppressor of RNA Silencing May Be Targeting a Plant Defence Pathway Involving Fibrillarin.Plants (Basel, Switzerland) · 2022Article
- Modification of the N-terminal FWKG-αH1 element of potyviral HC-Pro affects its multiple functions and generates effective attenuated mutants for cross-protection.Molecular plant pathology · 2022Article
- Effector-mediated plant-virus-vector interactions.The Plant cell · 2022Review
- Importin/exportin-mediated nucleocytoplasmic shuttling of cucumber mosaic virus 2b protein is required for 2b's efficient suppression of RNA silencing.PLoS pathogens · 2022Article
- Molecular signatures of silencing suppression degeneracy from a complex RNA virus.PLoS computational biology · 2021Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The multifunctional nuclear inclusion protein a (NIa) of potyviruses (genus Potyvirus; Potyviridae) accumulates in the nucleus of virus-infected cells for unknown reasons. In this study, two regions in the viral genome-linked protein (VPg) domain of NIa in Potato virus A (PVA) were found to constitute nuclear and nucleolar localization signals (NLS) in plant cells (Nicotiana spp). Amino acid substitutions in both NLS I (residues 4 to 9) and NLS II (residues 41 to 50) prevented nuclear localization, whereas mutations in either single NLS did not. Mutations in either NLS, however, prevented nucleolar localization and prevented or diminished virus replication in protoplasts, accumulation in infected plant tissues, and/or systemic movement in plants. One NLS mutant was partially complemented by the wild-type VPg expressed in transgenic plants. Furthermore, NLS I controlled NIa accumulation in Cajal bodies. The VPg domain interacted with fibrillarin, a nucleolar protein, and depletion of fibrillarin reduced PVA accumulation. Overexpression of VPg in leaf tissues interfered with cosuppression of gene expression (i.e., RNA silencing), whereas NLS I and NLS II mutants, which exhibited reduced nuclear and nucleolar localization, showed no such activity. These results demonstrate that some of the most essential viral functions required for completion of the infection cycle are tightly linked to regulation of the NIa nuclear and nucleolar localization.
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