ArticleAnalytical biochemistry2009
Generation of a natural glycan microarray using 9-fluorenylmethyl chloroformate (FmocCl) as a cleavable fluorescent tag.
Article in Analytical biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Serum antibody screening using glycan arrays.Chemical Society reviews · 2024Review
- Anti-schistosomal immunity to core xylose/fucose in N-glycans.Frontiers in molecular biosciences · 2023Article
- Examination of Whole-Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021Article
- Preparation of Complex Glycans From Natural Sources for Functional Study.Frontiers in chemistry · 2020Review
- Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder.MethodsX · 2019Article
- Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis.Bioconjugate chemistry · 2018Article
- Oxidative release of natural glycans for functional glycomics.Nature methods · 2016Article
- Fluorescently labelled glycans and their applications.Glycoconjugate journal · 2015Review
- Glycan microarrays of fluorescently-tagged natural glycans.Glycoconjugate journal · 2015Review
- Examination of whole cell galectin binding by solid phase and flow cytometric analysis.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Novel strategy to release and tag N-glycans for functional glycomics.Bioconjugate chemistry · 2014Article
- Molecular factors in dendritic cell responses to adsorbed glycoconjugates.Biomaterials · 2014Article
- Chemistry of natural glycan microarrays.Current opinion in chemical biology · 2014Review
- Carbohydrate microarrays.Chemical Society reviews · 2013Review
- Enhanced epimerization of glycosylated amino acids during solid-phase peptide synthesis.Journal of the American Chemical Society · 2012Article
- Analysis of influenza virus hemagglutinin receptor binding mutants with limited receptor recognition properties and conditional replication characteristics.Journal of virology · 2011Article
- Derivatization of free natural glycans for incorporation onto glycan arrays: derivatizing glycans on the microscale for microarray and other applications (ms# CP-10-0194).Current protocols in chemical biology · 2011Article
- Preparation and analysis of glycan microarrays.Current protocols in protein science · 2011Article
- Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: an update for 2009-2010.Mass spectrometry reviewsReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Glycan microarray technology has become a successful tool for studying protein-carbohydrate interactions, but a limitation has been the laborious synthesis of glycan structures by enzymatic and chemical methods. Here we describe a new method to generate quantifiable glycan libraries from natural sources by combining widely used protease digestion of glycoproteins and Fmoc chemistry. Glycoproteins including chicken ovalbumin, bovine fetuin, and horseradish peroxidase (HRP) were digested by Pronase, protected by FmocCl, and efficiently separated by 2D-HPLC. We show that glycans from HRP glycopeptides separated by HPLC and fluorescence monitoring retained their natural reducing end structures, mostly core alpha1,3-fucose and core alpha1,2-xylose. After simple Fmoc deprotection, the glycans were printed on NHS-activated glass slides. The glycans were interrogated using plant lectins and antibodies in sera from mice infected with Schistosoma mansoni, which revealed the presence of both IgM and IgG antibody responses to HRP glycopeptides. This simple approach to glycopeptide purification and conjugation allows for the development of natural glycopeptide microarrays without the need to remove and derivatize glycans and potentially compromise their reducing end determinants.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.