ArticleJournal of structural biology2009
Methods for identifying and averaging variable molecular conformations in tomograms of actively contracting insect flight muscle.
Article in Journal of structural biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Computational Methods Toward Unbiased Pattern Mining and Structure Determination in Cryo-Electron Tomography Data.Journal of molecular biology · 2023Review
- Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.International journal of molecular sciences · 2019Review
- Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts.Journal of structural biology · 2018Article
- Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography.Journal of structural biology · 2014Article
- Marker-free dual-axis tilt series alignment.Journal of structural biology · 2013Article
- A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography.Journal of structural biology · 2013Article
- Electron tomography of paracrystalline 2D arrays.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation.PloS one · 2012Article
- Interaction between troponin and myosin enhances contractile activity of myosin in cardiac muscle.DNA and cell biology · 2011Article
- Direct visualization of myosin-binding protein C bridging myosin and actin filaments in intact muscle.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography.Journal of molecular biology · 2010Article
- Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.PloS one · 2010Article
- Subtomogram alignment by adaptive Fourier coefficient thresholding.Journal of structural biology · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
During active muscle contraction, tension is generated through many simultaneous, independent interactions between the molecular motor myosin and the actin filaments. The ensemble of myosin motors displays heterogeneous conformations reflecting different mechanochemical steps of the ATPase pathway. We used electron tomography of actively contracting insect flight muscle fast-frozen, freeze substituted, Araldite embedded, thin-sectioned and stained, to obtain 3D snapshots of the multiplicity of actin-attached myosin structures. We describe procedures for alignment of the repeating lattice of sub-volumes (38.7 nm cross-bridge repeats bounded by troponin) and multivariate data analysis to identify self-similar repeats for computing class averages. Improvements in alignment and classification of repeat sub-volumes reveals (for the first time in active muscle images) the helix of actin subunits in the thin filament and the troponin density with sufficient clarity that a quasiatomic model of the thin filament can be built into the class averages independent of the myosin cross-bridges. We show how quasiatomic model building can identify both strong and weak myosin attachments to actin. We evaluate the accuracy of image classification to enumerate the different types of actin-myosin attachments.
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