ArticlePhotosynthesis research2009
A new setup for in vivo fluorescence imaging of photosynthetic activity.
Article in Photosynthesis research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
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- Individual and Interactive Effects of Temperature and Watering Regime on Canola Growth and Physiological Characteristics.Plant-environment interactions (Hoboken, N.J.) · 2025Article
- The stromal side of the cytochrome b6f complex regulates state transitions.The Plant cell · 2024Article
- Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism.The New phytologist · 2021Article
- Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the DiatomFrontiers in plant science · 2021Article
- A Genetic Screen to Identify New Molecular Players Involved in Photoprotection qH inPlants (Basel, Switzerland) · 2020Article
- Regulation of Light Harvesting inPlant physiology · 2020Article
- Stepwise Biogenesis of Subpopulations of Lipid Droplets in Nitrogen StarvedFrontiers in plant science · 2020Article
- The function of PROTOPORPHYRINOGEN IX OXIDASE in chlorophyll biosynthesis requires oxidised plastoquinone inCommunications biology · 2019Article
- Non-Enzymatic Synthesis of Bioactive Isoprostanoids in the DiatomPlant physiology · 2018Article
- Decoding β-Cyclocitral-Mediated Retrograde Signaling Reveals the Role of a Detoxification Response in Plant Tolerance to Photooxidative Stress.The Plant cell · 2018Article
- Dynamic Changes between Two LHCX-Related Energy Quenching Sites Control Diatom Photoacclimation.Plant physiology · 2018Article
- Screening for Biologically Annotated Drugs That Trigger Triacylglycerol Accumulation in the DiatomPlant physiology · 2018Article
- Structure-Function Analysis of Chloroplast Proteins via Random Mutagenesis Using Error-Prone PCR.Plant physiology · 2018Article
- The Plastid Lipocalin LCNP Is Required for Sustained Photoprotective Energy Dissipation in Arabidopsis.The Plant cell · 2018Article
- A stromal region of cytochromeProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Nitric Oxide Mediates Nitrite-Sensing and Acclimation and Triggers a Remodeling of Lipids.Plant physiology · 2017Article
- Investigating mixotrophic metabolism in the model diatomPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
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Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe a new imaging setup able to assess in vivo photosynthetic activity. The system specifically measures time-resolved chlorophyll fluorescence in response to light. It is composed of a fast digital camera equipped with a wide-angle lens for the analysis of samples up to 10 x 10 cm, i.e. entire plants or petri dishes. In the choice of CCD, we have opted for a 12-bits high frame rate [150 fps (frames per second)] at the expense of definition (640 x 480 pixels). Although the choice of digital camera is always a compromise between these two related features, we have designed a flexible system allowing the fast sampling of images (down to 100 micros) with a maximum spatial resolution. This image readout system, synchronized with actinic light and saturating pulses, allows a precise determination of F(0) and F(M), which is required to monitor PSII activity. This new imaging system, together with image processing techniques, is useful to investigate the heterogeneity of photosynthetic activity within leaves or to screen large numbers of unicellular algal mutant colonies to identify those with subtle changes in photosynthetic electron flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.