ArticleJournal of biomedicine & biotechnology2009
Induction of tetraploids from petiole explants through colchicine treatments in Echinacea purpurea L.
Article in Journal of biomedicine & biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 75 citations in OpenAlex.
- In vitro synthetic polyploidization and enhancement of anticancer compounds in Catharanthus Roseus (L.) G. Don important cultivars.Scientific reports · 2025Article
- Induction of tetraploids in Paper Mulberry (Broussonetia papyrifera (L.) L'Hér. ex Vent.) by colchicine.BMC plant biology · 2023Article
- In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis.BMC plant biology · 2023Article
- In vitro octaploid induction of Populus hopeiensis with colchicine.BMC plant biology · 2022Article
- Comparative Transcriptomic, Anatomical and Phytohormone Analyses Provide New Insights Into Hormone-Mediated Tetraploid Dwarfing in Hybrid Sweetgum (Frontiers in plant science · 2022Article
- Article
- Review
- Echinacea biotechnology: advances, commercialization and future considerations.Pharmaceutical biology · 2018Review
- Chromosome Doubling-Enhanced Biomass and Dihydrotanshinone I Production in Salvia miltiorrhiza, A Traditional Chinese Medicinal Plant.Molecules (Basel, Switzerland) · 2018Article
- A Comparison of the Immunostimulatory Effects of Polysaccharides from Tetraploid and DiploidBioMed research international · 2018Article
- Enhanced Agronomic Traits and Medicinal Constituents of Autotetraploids in Anoectochilus formosanus Hayata, a Top-Grade Medicinal Orchid.Molecules (Basel, Switzerland) · 2017Article
- Article
- An uncommon plant growth regulator, diethyl aminoethyl hexanoate, is highly effective in tissue cultures of the important medicinal plant purple coneflower (Echinacea purpurea L.).BioMed research international · 2013Article
- In vitro tetraploid induction from leaf explants of Populus pseudo-simonii Kitag.Plant cell reports · 2011Article
- Polyploidization facilitates biotechnological in vitro techniques in the genus Cucumis.Journal of biomedicine & biotechnology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Petiole explants were obtained from in vitro grown diploid (2x = 22) Echinacea purpurea plantlets. Shoots were regenerated by culturing the explants on MS basal medium containing 0.3 mg/L benzyladenine (BA), 0.01 mg/L naphthaleneacetic acid (NAA) and four concentrations (30, 60, 120, and 240 mg/L) of colchicine for 30 days, or 120 mg/L of colchicine for various durations (7, 14, 21, and 28 days). The regenerated shoots were induced to root on MS basal medium with 0.01 mg/L NAA, and then the root-tips of the regenerated shoots were sampled for count of chromosome number. It was found that a treatment duration of >7 days was necessary for induction of tetraploid (4x = 44) shoots, and treatment with 120 mg/L colchicine for 28 days was the most efficient for induction of tetraploids, yielding 23.5% of tetraploids among all the regenerated shoots. Chimeras were observed in almost all the treatments. However, the ratio of tetraploid to diploid cells in a chimeric plant was usually low. In comparison with diploid plants, tetraploid plants in vitro had larger stomata and thicker roots with more root branches, and had prominently shorter inflorescence stalk when mature.
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