ArticleCirculation research2009
iPS programmed without c-MYC yield proficient cardiogenesis for functional heart chimerism.
Article in Circulation research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
63 citing papers in PubMed, 99 citations in OpenAlex.
- Chromatin accessibility dynamics during cell fate reprogramming.EMBO reports · 2021Review
- Emerging roles of Myc in stem cell biology and novel tumor therapies.Journal of experimental & clinical cancer research : CR · 2018Review
- The march of pluripotent stem cells in cardiovascular regenerative medicine.Stem cell research & therapy · 2018Review
- The present and future role of ultrasound targeted microbubble destruction in preclinical studies of cardiac gene therapy.Journal of thoracic disease · 2018Review
- Cell culture density affects the stemness gene expression of adipose tissue-derived mesenchymal stem cells.Biomedical reports · 2017Article
- Pharmacological Modulation of Calcium Homeostasis in Familial Dilated Cardiomyopathy: An In Vitro Analysis From an RBM20 Patient-Derived iPSC Model.Clinical and translational science · 2016Article
- Generation of electrophysiologically functional cardiomyocytes from mouse induced pluripotent stem cells.Stem cell research · 2016Article
- MicroRNAs and Cardiac Regeneration.Circulation research · 2015Review
- Cardiac regeneration and diabetes.Regenerative medicine research · 2014Review
- iPS cell-derived cardiogenicity is hindered by sustained integration of reprogramming transgenes.Circulation. Cardiovascular genetics · 2014Article
- Selection via pluripotency-related transcriptional screen minimizes the influence of somatic origin on iPSC differentiation propensity.Stem cells (Dayton, Ohio) · 2014Article
- Therapeutic effects of induced pluripotent stem cells in chimeric mice with β-thalassemia.Haematologica · 2014Article
- The current status of iPS cells in cardiac research and their potential for tissue engineering and regenerative medicine.Stem cell reviews and reports · 2014Article
- Preclinical studies for induced pluripotent stem cell-based therapeutics.The Journal of biological chemistry · 2014Review
- Natural cardiogenesis-based template predicts cardiogenic potential of induced pluripotent stem cell lines.Circulation. Cardiovascular genetics · 2013Article
- Stem cell therapy for electrophysiological disorders.Current cardiology reports · 2013Review
- Induced pluripotent stem cell intervention rescues ventricular wall motion disparity, achieving biological cardiac resynchronization post-infarction.The Journal of physiology · 2013Article
- Endogenous cardiac stem cells for the treatment of heart failure.Stem cells and cloning : advances and applications · 2013Review
- Nuclear reprogramming with c-Myc potentiates glycolytic capacity of derived induced pluripotent stem cells.Journal of cardiovascular translational research · 2013Article
- Stimulation of adult resident cardiac progenitor cells by durable myocardial expression of thymosin beta 4 with ultrasound-targeted microbubble delivery.Gene therapy · 2013Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
rationaleInduced pluripotent stem cells (iPS) allow derivation of pluripotent progenitors from somatic sources. Originally, iPS were induced by a stemness-related gene set that included the c-MYC oncogene.
objectiveHere, we determined from embryo to adult the cardiogenic proficiency of iPS programmed without c-MYC, a cardiogenicity-associated transcription factor. METHODS AND
resultsTransgenic expression of 3 human stemness factors SOX2, OCT4, and KLF4 here reset murine fibroblasts to the pluripotent ground state. Transduction without c-MYC reversed cellular ultrastructure into a primitive archetype and induced stem cell markers generating 3-germ layers, all qualifiers of acquired pluripotency. Three-factor induced iPS (3F-iPS) clones reproducibly demonstrated cardiac differentiation properties characterized by vigorous beating activity of embryoid bodies and robust expression of cardiac Mef2c, alpha-actinin, connexin43, MLC2a, and troponin I. In vitro isolated iPS-derived cardiomyocytes demonstrated functional excitation-contraction coupling. Chimerism with 3F-iPS derived by morula-stage diploid aggregation was sustained during prenatal heart organogenesis and contributed in vivo to normal cardiac structure and overall performance in adult tumor-free offspring.
conclusionsThus, 3F-iPS bioengineered without c-MYC achieve highest stringency criteria for bona fide cardiogenesis enabling reprogrammed fibroblasts to yield de novo heart tissue compatible with native counterpart throughout embryological development and into adulthood.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.