ArticleMolecular & cellular proteomics : MCP2009
Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides small colony type.
Article in Molecular & cellular proteomics : MCP, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Protein-specific analysis of humoral immune responses in a clinical trial for vaccines against contagious bovine pleuropneumonia.Clinical and vaccine immunology : CVI · 2010Trial
- Review and comprehensive analysis of knowledge, tools, and implementation gaps for the control of contagious bovine pleuropneumonia.BMC veterinary research · 2025Review
- Antibodies to Cartilage Oligomeric Matrix Protein Are Pathogenic in Mice and May Be Clinically Relevant in Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2022Article
- Influence of hydrocarbon oil structure on adjuvanticity and autoimmunity.Scientific reports · 2017Article
- Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia.Journal of clinical microbiology · 2016Article
- Development of an improved vaccine for contagious bovine pleuropneumonia: an African perspective on challenges and proposed actions.Veterinary research · 2013Article
- Autoantibody profiling in multiple sclerosis using arrays of human protein fragments.Molecular & cellular proteomics : MCP · 2013Article
- Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.PloS one · 2013Article
- Specific evolution of F1-like ATPases in mycoplasmas.PloS one · 2012Article
- A lateral flow protein microarray for rapid and sensitive antibody assays.International journal of molecular sciences · 2011Article
- Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens.Clinical and vaccine immunology : CVI · 2010Article
- A versatile palindromic amphipathic repeat coding sequence horizontally distributed among diverse bacterial and eucaryotic microbes.BMC genomics · 2010Article
- Multiplex screening of surface proteins from Mycoplasma mycoides subsp. mycoides small colony for an antigen cocktail enzyme-linked immunosorbent assay.Clinical and vaccine immunology : CVI · 2009Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A systematic approach to characterize the surface proteome of Mycoplasma mycoides subspecies mycoides small colony type (M. mycoides SC), the causative agent of contagious bovine pleuropneumonia (CBPP) in cattle, is presented. Humoral immune responses in 242 CBPP-affected cattle and controls were monitored against one-third of the surface proteins of M. mycoides SC in a high throughput magnetic bead-based assay. Initially, 64 surface proteins were selected from the genome sequence of M. mycoides SC and expressed as recombinant proteins in Escherichia coli. Binding of antibodies to each individual protein could then be analyzed simultaneously in minute sample volumes with the Luminex suspension array technology. The assay was optimized on Namibian CBPP-positive sera and Swedish negative controls to allow detection and 20-fold mean signal separation between CBPP-positive and -negative sera. Signals were proven to be protein-specific by inhibition experiments, and results agreed with Western blot experiments. The potential of the assay to monitor IgG, IgM, and IgA responses over time was shown in a proof-of-concept study with 116 sera from eight animals in a CBPP vaccine study. In conclusion, a toolbox with recombinant proteins and a flexible suspension array assay that allows multiplex analysis of humoral immune responses to M. mycoides SC has been created.
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