ArticleThe Journal of biological chemistry2009
Prolyl 4-hydroxylation of alpha-fibrinogen: a novel protein modification revealed by plasma proteomics.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Trial
- The unseen architects of metastasis: coagulation factors in pre-metastatic niche development.Cell communication and signaling : CCS · 2026Review
- Single-EV analysis (sEVA) of mutated proteins allows detection of stage 1 pancreatic cancer.Science advances · 2022Article
- Targeted proteomics for cancer biomarker verification and validation.Cancer biomarkers : section A of Disease markers · 2022Review
- Label-Free Method Development for Hydroxyproline PTM Mapping in Human Plasma Proteome.The protein journal · 2021Article
- Molecular Dynamic Simulations Suggest That Metabolite-Induced Post-Translational Modifications Alter the Behavior of the Fibrinogen Coiled-Coil Domain.Metabolites · 2021Article
- Article
- Nuclear receptor/Wnt beta-catenin interactions are regulated via differential CBP/p300 coactivator usage.PloS one · 2018Article
- PTPRQ as a potential biomarker for idiopathic normal pressure hydrocephalus.Molecular medicine reports · 2017Article
- Comparative proteome analysis of human esophageal cancer and adjacent normal tissues.Iranian journal of basic medical sciences · 2017Article
- Potential usefulness of apolipoprotein A2 isoforms for screening and risk stratification of pancreatic cancer.Biomarkers in medicine · 2016Review
- Plasma biomarker for detection of early stage pancreatic cancer and risk factors for pancreatic malignancy using antibodies for apolipoprotein-AII isoforms.Scientific reports · 2015Article
- Article
- Mps1 phosphorylation of condensin II controls chromosome condensation at the onset of mitosis.The Journal of cell biology · 2014Article
- Galectin-7 as a potential predictive marker of chemo- and/or radio-therapy resistance in oral squamous cell carcinoma.Cancer medicine · 2014Article
- GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.Nature communications · 2013Article
- Ribophorin II regulates breast tumor initiation and metastasis through the functional suppression of GSK3β.Scientific reports · 2013Article
- Biomarker Discovery of Pancreatic and Gastrointestinal Cancer by 2DICAL: 2-Dimensional Image-Converted Analysis of Liquid Chromatography and Mass Spectrometry.International journal of proteomics · 2012Article
- Carbonic anhydrase I as a new plasma biomarker for prostate cancer.ISRN oncology · 2012Article
- Article
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Authors and funding
24 authors at 9 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasma proteome analysis requires sufficient power to compare numerous samples and detect changes in protein modification, because the protein content of human samples varies significantly among individuals, and many plasma proteins undergo changes in the bloodstream. A label-free proteomics platform developed in our laboratory, termed "Two-Dimensional Image Converted Analysis of Liquid chromatography and mass spectrometry (2DICAL)," is capable of these tasks. Here, we describe successful detection of novel prolyl hydroxylation of alpha-fibrinogen using 2DICAL, based on comparison of plasma samples of 38 pancreatic cancer patients and 39 healthy subjects. Using a newly generated monoclonal antibody 11A5, we confirmed the increase in prolyl-hydroxylated alpha-fibrinogen plasma levels and identified prolyl 4-hydroxylase A1 as a key enzyme for the modification. Competitive enzyme-linked immunosorbent assay of 685 blood samples revealed dynamic changes in prolyl-hydroxylated alpha-fibrinogen plasma level depending on clinical status. Prolyl-hydroxylated alpha-fibrinogen is presumably controlled by multiple biological mechanisms, which remain to be clarified in future studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.