ArticleThe Journal of biological chemistry2009
The ubiquitin-like protein LC3 regulates the Rho-GEF activity of AKAP-Lbc.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signaling.Nature communications · 2025Article
- Beyond self‑eating: Emerging autophagy‑independent functions for the autophagy molecules in cancer (Review).International journal of oncology · 2024Review
- To eat or not to eat: a critical review on the role of autophagy in prostate carcinogenesis and prostate cancer therapeutics.Frontiers in pharmacology · 2024Review
- Role of ATG7-dependent non-autophagic pathway in angiogenesis.Frontiers in pharmacology · 2023Review
- NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1.The Journal of biological chemistry · 2020Article
- Spatiotemporal regulation of autophagy duringeLife · 2017Article
- AKAP150 participates in calcineurin/NFAT activation during the down-regulation of voltage-gated K(+) currents in ventricular myocytes following myocardial infarction.Cellular signalling · 2016Article
- Atg8 is involved in endosomal and phagosomal acidification in the parasitic protist Entamoeba histolytica.Cellular microbiology · 2015Article
- Autophagy and SQSTM1 on the RHOA(d) again: emerging roles of autophagy in the degradation of signaling proteins.Autophagy · 2014Article
- The MLL recombinome of acute leukemias in 2013.Leukemia · 2013Article
- Non-autophagic roles of autophagy-related proteins.EMBO reports · 2013Review
- Streamlined embedding of cell monolayers on gridded glass-bottom imaging dishes for correlative light and electron microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2010Article
- LC3-dependent intracellular membrane tubules induced by gamma-protocadherins A3 and B2: a role for intraluminal interactions.The Journal of biological chemistry · 2010Article
- Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Networking with AKAPs: context-dependent regulation of anchored enzymes.Molecular interventions · 2010Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AKAP-Lbc is a member of the A-kinase anchoring protein (AKAP) family that has been recently associated with the development of pathologies, such as cardiac hypertrophy and cancer. We have previously demonstrated that, at the molecular level, AKAP-Lbc functions as a guanine nucleotide exchange factor (GEF) that promotes the specific activation of RhoA. In the present study, we identified the ubiquitin-like protein LC3 as a novel regulatory protein interacting with AKAP-Lbc. Mutagenesis studies revealed that LC3, through its NH(2)-terminal alpha-helical domain, interacts with two binding sites located within the NH(2)-terminal regulatory region of AKAP-Lbc. Interestingly, LC3 overexpression strongly reduced the ability of AKAP-Lbc to interact with RhoA, profoundly impairing the Rho-GEF activity of the anchoring protein and, as a consequence, its ability to promote cytoskeletal rearrangements associated with the formation of actin stress fibers. Moreover, AKAP-Lbc mutants that fail to interact with LC3 show a higher basal Rho-GEF activity as compared with the wild type protein and become refractory to the inhibitory effect of LC3. This suggests that LC3 binding maintains AKAP-Lbc in an inactive state that displays a reduced ability to promote downstream signaling. Collectively, these findings provide evidence for a previously uncharacterized role of LC3 in the regulation of Rho signaling and in the reorganization of the actin cytoskeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.