ArticleVirology2009
The African swine fever virus g5R protein possesses mRNA decapping activity.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Hybrid genotype I and II ASFVEmerging microbes & infections · 2026Article
- Role of g5Rp in African swine fever virus replication: disruption of host translation and autophagy.Journal of virology · 2026Article
- Biochemical and functional characterization of orf virus decapping protein OV71.Virology journal · 2025Article
- Article
- InsPection of electron density maps supports wrongly modeled hexakisphosphate (InsP6) bound to African swine fever mRNA-decapping enzyme g5Rp.Journal of virology · 2024Article
- Article
- Structure and function of African swine fever virus proteins: Current understanding.Frontiers in microbiology · 2023Review
- The Role of Viral RNA Degrading Factors in Shutoff of Host Gene Expression.Annual review of virology · 2022Review
- Apprehending the NADViruses · 2022Article
- Article
- Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9.ACS chemical biology · 2022Article
- Structural Insight into Molecular Inhibitory Mechanism of InsPJournal of virology · 2022Article
- A novel function of African Swine Fever Virus pE66L in inhibition of host translation by the PKR/eIF2α pathway.Journal of virology · 2021Article
- The Mimivirus L375 Nudix enzyme hydrolyzes the 5' mRNA cap.PloS one · 2021Article
- It's the Little Things (in Viral RNA).mBio · 2020Review
- Viral-Mediated mRNA Degradation for Pathogenesis.Biomedicines · 2018Review
- Viral manipulation of host mRNA decay.Future virology · 2018Article
- Characterization of the African Swine Fever Virus Decapping Enzyme during Infection.Journal of virology · 2017Article
- BA71ΔCD2: a New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities.Journal of virology · 2017Article
- One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions.Genome biology and evolution · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
The African Swine Fever Virus (ASFV) encodes a single Nudix enzyme in its genome, termed the g5R protein (g5Rp). Nudix phosphohydrolases cleave a variety of substrates, such as nucleotides and diphosphoinositol polyphosphates. Previously, ASFV g5Rp was shown to hydrolyze diphosphoinositol polyphosphates and GTP, but was unable to cleave methylated mRNA cap analogues. In vaccinia virus (VACV), a distant relative of ASFV, the D9 and D10 Nudix enzymes were shown to cleave the mRNA cap, but only when the cap was attached to an RNA body. Here, we show that recombinant ASFV g5Rp hydrolyzes the mRNA cap when tethered to an RNA moiety, liberating m(7)GDP as a product. Mutations in the Nudix motif abolished mRNA decapping activity, confirming that g5Rp was responsible for cap cleavage. The decapping activity of g5Rp was potently inhibited by excess uncapped RNA but not by methylated cap analogues, suggesting that substrate recognition occurs by RNA binding.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.