ArticleExperimental eye research2009
Microtubule stabilization opposes the (TNF-alpha)-induced loss in the barrier integrity of corneal endothelium.
Article in Experimental eye research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Quantitative imaging of corneal endothelial development reveals dynamic but resilient monolayer.bioRxiv : the preprint server for biology · 2026Article
- Molecular Motors in Myelination and Their Misregulation in Disease.Molecular neurobiology · 2025Review
- Selective HDAC6 inhibition protects against blood-brain barrier dysfunction after intracerebral hemorrhage.CNS neuroscience & therapeutics · 2024Article
- Fusogenic liposome-coated nanoparticles for rapid internalization into donor corneal endothelial tissue to enable prophylaxis before transplantation.Nanoscale advances · 2023Article
- Isolation efficiency of collagenase and EDTA for the culture of corneal endothelial cells.Molecular vision · 2022Article
- Role of HDAC6 inhibition in sepsis-induced acute respiratory distress syndrome (Review).Experimental and therapeutic medicine · 2021Review
- Potential Factors of Corneal Endothelial Cells for Progression in Children with Uveitis.Disease markers · 2021Article
- Microtubules as Major Regulators of Endothelial Function: Implication for Lung Injury.Frontiers in physiology · 2021Review
- Essential role of ultraviolet radiation in the decrease of corneal endothelial cell density caused by pterygium.Eye (London, England) · 2018Article
- Axonal transport deficits in multiple sclerosis: spiraling into the abyss.Acta neuropathologica · 2017Review
- Role of End Binding Protein-1 in endothelial permeability response to barrier-disruptive and barrier-enhancing agonists.Cellular signalling · 2017Article
- Cytotoxic activity and degradation patterns of structural proteins by corneal isolates of Acanthamoeba spp.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2015Article
- Role of microtubules in attenuation of PepG-induced vascular endothelial dysfunction by atrial natriuretic peptide.Biochimica et biophysica acta · 2015Article
- Control of vascular permeability by atrial natriuretic peptide via a GEF-H1-dependent mechanism.The Journal of biological chemistry · 2014Article
- The leading role of microtubules in endothelial barrier dysfunction: disassembly of peripheral microtubules leaves behind the cytoskeletal reorganization.Journal of cellular biochemistry · 2013Article
- Normal IgG downregulates the intracellular superoxide level and attenuates migration and permeability in human aortic endothelial cells isolated from a hypertensive patient.Hypertension (Dallas, Tex. : 1979) · 2012Article
- Cell signaling in regulation of the barrier integrity of the corneal endothelium.Experimental eye research · 2012Review
- Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers.PloS one · 2012Article
- Rho-Rho kinase pathway in the actomyosin contraction and cell-matrix adhesion in immortalized human trabecular meshwork cells.Molecular vision · 2011Article
- Elevated cAMP opposes (TNF-alpha)-induced loss in the barrier integrity of corneal endothelium.Molecular vision · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule disassembly breaks down the barrier integrity in a number of epithelial and endothelial monolayers. This study has investigated effects of TNF-alpha, which is implicated in corneal allograft rejection, on microtubules and barrier integrity in cultured bovine corneal endothelial cells. Exposure to TNF-alpha led to disassembly of the microtubules, and also caused disruption of the perijunctional actomyosin ring (PAMR). As a measure of barrier integrity, trans-endothelial electrical resistance (TER) was determined based on electrical cell-substrate impedance sensing in realtime. Exposure to TNF-alpha caused a slow decline in TER for > 20 h, and a similar exposure to cells grown on porous culture inserts led to a significant increase in permeability to FITC dextran. These changes, indicating a loss of barrier integrity, were also reflected by dislocation of ZO-1 at the cell border and disassembly of cadherins. These effects of TNF-alpha were inhibited upon stabilization of microtubules by pre-treatment with paclitaxel or epothilone B. Microtubule stabilization may be a useful strategy to overcome (TNF-alpha)-induced loss of the barrier integrity of corneal endothelium during inflammation associated with transplant rejection and uveitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.