ArticleBMC plant biology2009
Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 39 citations in OpenAlex.
- Mutations in PmUFGT3 contribute to color variation of fruit skin in Japanese apricot (Prunus mume Sieb. et Zucc.).BMC plant biology · 2022Article
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- Biosynthesis of the anti-diabetic metabolite montbretin A: glucosylation of the central intermediate mini-MbA.The Plant journal : for cell and molecular biology · 2019Article
- Candidate Enzymes for Saffron Crocin Biosynthesis Are Localized in Multiple Cellular Compartments.Plant physiology · 2018Article
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- Functional Characterization ofThe Journal of biological chemistry · 2017Article
- Transcriptome-Guided Mining of Genes Involved in Crocin Biosynthesis.Frontiers in plant science · 2017Article
- Crocins with high levels of sugar conjugation contribute to the yellow colours of early-spring flowering crocus tepals.PloS one · 2013Article
- Characterization of a glucosyltransferase enzyme involved in the formation of kaempferol and quercetin sophorosides in Crocus sativus.Plant physiology · 2012Article
- Article
- Saffron'omics': The challenges of integrating omic technologies.Avicenna journal of phytomedicineReview
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundFlavonol glucosides constitute the second group of secondary metabolites that accumulate in Crocus sativus stigmas. To date there are no reports of functionally characterized flavonoid glucosyltransferases in C. sativus, despite the importance of these compounds as antioxidant agents. Moreover, their bitter taste makes them excellent candidates for consideration as potential organoleptic agents of saffron spice, the dry stigmas of C. sativus.
resultsUsing degenerate primers designed to match the plant secondary product glucosyltransferase (PSPG) box we cloned a full length cDNA encoding CsGT45 from C. sativus stigmas. This protein showed homology with flavonoid glucosyltransferases. In vitro reactions showed that CsGT45 catalyses the transfer of glucose from UDP_glucose to kaempferol and quercetin. Kaempferol is the unique flavonol present in C. sativus stigmas and the levels of its glucosides changed during stigma development, and these changes, are correlated with the expression levels of CsGT45 during these developmental stages.
conclusionFindings presented here suggest that CsGT45 is an active enzyme that plays a role in the formation of flavonoid glucosides in C. sativus.
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