ArticleThe FEBS journal2009
A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+.
Article in The FEBS journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Nox1-Derived ROS Amplifies Calcium Entry and Enhances Pneumolysin-Induced Lung Endothelial Barrier Dysfunction in Hyperglycemia.Antioxidants (Basel, Switzerland) · 2026Article
- Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and function.Genetics · 2025Article
- Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and function.bioRxiv : the preprint server for biology · 2025Article
- High-throughput identification of calcium-regulated proteins across diverse proteomes.Cell reports · 2024Article
- High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes.bioRxiv : the preprint server for biology · 2024Article
- Recognition ofBiology · 2024Article
- Alteration of STIM1/Orai1-Mediated SOCE in Skeletal Muscle: Impact in Genetic Muscle Diseases and Beyond.Cells · 2021Review
- The leucine zipper EF-hand containing transmembrane protein-1 EF-hand is a tripartite calcium, temperature, and pH sensor.Protein science : a publication of the Protein Society · 2021Article
- Functional consequences of enhanced expression of STIM1 and Orai1 in Huh-7 hepatocellular carcinoma tumor-initiating cells.BMC cancer · 2019Article
- Discovery of Small-Molecule Inhibitors of the HSP90-Calcineurin-NFAT Pathway against Glioblastoma.Cell chemical biology · 2019Article
- Identification of molecular determinants that govern distinct STIM2 activation dynamics.PLoS biology · 2018Article
- Calcium sensing by the STIM1 ER-luminal domain.Nature communications · 2018Article
- Molecular Basis for Modulation of Metabotropic Glutamate Receptors and Their Drug Actions by Extracellular CaInternational journal of molecular sciences · 2017Review
- STIM1 Phosphorylation at Y361 Recruits Orai1 to STIM1 Puncta and Induces CaScientific reports · 2017Article
- Stromal Interaction Molecule 1 (STIM1) Regulates ATP-sensitive Potassium (The Journal of biological chemistry · 2017Article
- Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation.Journal of cell science · 2016Review
- Store-Operated Calcium Channels.Physiological reviews · 2015Review
- Calciomics: integrative studies of Ca2+-binding proteins and their interactomes in biological systems.Metallomics : integrated biometal science · 2013Review
- Modulation of STIM1 and capacitative Ca2+ entry by the endoplasmic reticulum luminal oxidoreductase ERp57.EMBO reports · 2011Article
- Molecular basis of calcium signaling in lymphocytes: STIM and ORAI.Annual review of immunology · 2010Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Stromal interaction molecule 1 (STIM1) is responsible for activating the Ca(2+) release-activated Ca(2+) (CRAC) channel by first sensing the changes in Ca(2+) concentration in the endoplasmic reticulum ([Ca(2+)](ER)) via its luminal canonical EF-hand motif and subsequently oligomerizing to interact with the CRAC channel pore-forming subunit Orai1. In this work, we applied a grafting approach to obtain the intrinsic metal-binding affinity of the isolated EF-hand of STIM1, and further investigated its oligomeric state using pulsed-field gradient NMR and size-exclusion chromatography. The canonical EF-hand bound Ca(2+) with a dissociation constant at a level comparable with [Ca(2+)](ER) (512 +/- 15 microm). The binding of Ca(2+) resulted in a more compact conformation of the engineered protein. Our results also showed that D to A mutations at Ca(2+)-coordinating loop positions 1 and 3 of the EF-hand from STIM1 led to a 15-fold decrease in the metal-binding affinity, which explains why this mutant was insensitive to changes in Ca(2+) concentration in the endoplasmic reticulum ([Ca(2+)](ER)) and resulted in constitutive punctae formation and Ca(2+) influx. In addition, the grafted single EF-hand motif formed a dimer regardless of the presence of Ca(2+), which conforms to the EF-hand paring paradigm. These data indicate that the STIM1 canonical EF-hand motif tends to dimerize for functionality in solution and is responsible for sensing changes in [Ca(2+)](ER).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.