ArticleEnvironmental microbiology2009
Culturable prokaryotic diversity of deep, gas hydrate sediments: first use of a continuous high-pressure, anaerobic, enrichment and isolation system for subseafloor sediments (DeepIsoBUG).
Article in Environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 104 citations in OpenAlex.
- Moderate heating renders 7.8-million-year-old sedimentary organic matter bioavailable.Science advances · 2025Article
- High-pressure continuous culturing: life at the extreme.Applied and environmental microbiology · 2025Review
- Microfluidics for studying the deep underground biosphere: from applications to fundamentals.FEMS microbiology ecology · 2024Review
- Article
- Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation.Scientific reports · 2019Article
- Microbial dormancy in the marine subsurface: Global endospore abundance and response to burial.Science advances · 2019Article
- Pressure-Retaining Sampler and High-Pressure Systems to Study Deep-Sea Microbes UnderFrontiers in microbiology · 2019Article
- Hydrostatic Pressure Helps to Cultivate an Original Anaerobic Bacterium From the Atlantis Massif Subseafloor (IODP Expedition 357):Frontiers in microbiology · 2019Article
- Review
- Response of marine bacteria to oil contamination and to high pressure and low temperature deep sea conditions.MicrobiologyOpen · 2018Article
- Response of Bacterial Communities to Different Detritus Compositions in Arctic Deep-Sea Sediments.Frontiers in microbiology · 2017Article
- Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment.Frontiers in microbiology · 2017Article
- Whole-genome sequence of Sunxiuqinia dokdonensis DH1(T), isolated from deep sub-seafloor sediment in Dokdo Island.Genomics data · 2016Article
- Co-occurrence of Methanosarcina mazei and Geobacteraceae in an iron (III)-reducing enrichment culture.Frontiers in microbiology · 2015Article
- Temperature and pressure adaptation of a sulfate reducer from the deep subsurface.Frontiers in microbiology · 2015Article
- Stratified community responses to methane and sulfate supplies in mud volcano deposits: insights from an in vitro experiment.PloS one · 2014Article
- Iron reduction by the deep-sea bacterium Shewanella profunda LT13a under subsurface pressure and temperature conditions.Frontiers in microbiology · 2014Article
- Trends and future challenges in sampling the deep terrestrial biosphere.Frontiers in microbiology · 2014Article
- Sunxiuqinia dokdonensis sp. nov., isolated from deep sub-seafloor sediment.Journal of microbiology (Seoul, Korea) · 2013Article
- Microbial activity in the marine deep biosphere: progress and prospects.Frontiers in microbiology · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Deep subseafloor sediments may contain depressurization-sensitive, anaerobic, piezophilic prokaryotes. To test this we developed the DeepIsoBUG system, which when coupled with the HYACINTH pressure-retaining drilling and core storage system and the PRESS core cutting and processing system, enables deep sediments to be handled without depressurization (up to 25 MPa) and anaerobic prokaryotic enrichments and isolation to be conducted up to 100 MPa. Here, we describe the system and its first use with subsurface gas hydrate sediments from the Indian Continental Shelf, Cascadia Margin and Gulf of Mexico. Generally, highest cell concentrations in enrichments occurred close to in situ pressures (14 MPa) in a variety of media, although growth continued up to at least 80 MPa. Predominant sequences in enrichments were Carnobacterium, Clostridium, Marinilactibacillus and Pseudomonas, plus Acetobacterium and Bacteroidetes in Indian samples, largely independent of media and pressures. Related 16S rRNA gene sequences for all of these Bacteria have been detected in deep, subsurface environments, although isolated strains were piezotolerant, being able to grow at atmospheric pressure. Only the Clostridium and Acetobacterium were obligate anaerobes. No Archaea were enriched. It may be that these sediment samples were not deep enough (total depth 1126-1527 m) to obtain obligate piezophiles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.