ArticleProtein science : a publication of the Protein Society2009
Structure-based design of novel Chlamydomonas reinhardtii D1-D2 photosynthetic proteins for herbicide monitoring.
Article in Protein science : a publication of the Protein Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 62 citations in OpenAlex.
- Review
- Effects of atrazine and S-metolachlor on stream periphyton taxonomic and fatty acid compositions.Ecotoxicology (London, England) · 2024Article
- An All-Green Photo-Electrochemical Biosensor Using Microalgae Immobilized on Eco-Designed Lignin-Based Screen-Printed Electrodes to Detect Sustainable Nanoherbicides.International journal of molecular sciences · 2023Article
- Electrochemical biosensors in healthcare services: bibliometric analysis and recent developments.PeerJ · 2023Review
- Ameliorative effects of endogenous and exogenous indole-3-acetic acid on atrazine stressed paddy field cyanobacterial biofertilizer Cylindrospermum stagnale.Scientific reports · 2022Article
- Recent Advances in Electrochemical Biosensors: Applications, Challenges, and Future Scope.Biosensors · 2021Review
- Physiological and Molecular Analysis Reveals the Differences of Photosynthesis between Colored and Green Leaf Poplars.International journal of molecular sciences · 2021Article
- Binding Properties of Photosynthetic Herbicides with the QPlants (Basel, Switzerland) · 2021Article
- Dynamics Properties of Photosynthetic Microorganisms Probed by Incoherent Neutron Scattering.Biophysical journal · 2019Article
- Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QScientific reports · 2018Article
- Evaluation of a biohybrid photoelectrochemical cell employing the purple bacterial reaction centre as a biosensor for herbicides.Biosensors & bioelectronics · 2014Article
- Structure/function/dynamics of photosystem II plastoquinone binding sites.Current protein & peptide science · 2014Review
- Photosynthesis at the forefront of a sustainable life.Frontiers in chemistry · 2014Review
- A powerful molecular engineering tool provided efficient Chlamydomonas mutants as bio-sensing elements for herbicides detection.PloS one · 2013Article
- Article
- Redesign of the Chlamydomonas reinhardtii QPhysiologia plantarumArticle
- Biosensors and their applications - A review.Journal of oral biology and craniofacial researchReview
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The D1-D2 heterodimer in the reaction center core of phototrophs binds the redox plastoquinone cofactors, Q(A) and Q(B), the terminal acceptors of the photosynthetic electron transfer chain in the photosystem II (PSII). This complex is the target of the herbicide atrazine, an environmental pollutant competitive inhibitor of Q(B) binding, and consequently it represents an excellent biomediator to develop biosensors for pollutant monitoring in ecosystems. In this context, we have undertaken a study of the Chlamydomonas reinhardtii D1-D2 proteins aimed at designing site directed mutants with increased affinity for atrazine. The three-dimensional structure of the D1 and D2 proteins from C. reinhardtii has been homology modeled using the crystal structure of the highly homologous Thermosynechococcus elongatus proteins as templates. Mutants of D1 and D2 were then generated in silico and the atrazine binding affinity of the mutant proteins has been calculated to predict mutations able to increase PSII affinity for atrazine. The computational approach has been validated through comparison with available experimental data and production and characterization of one of the predicted mutants. The latter analyses indicated an increase of one order of magnitude of the mutant sensitivity and affinity for atrazine as compared to the control strain. Finally, D1-D2 heterodimer mutants were designed and selected which, according to our model, increase atrazine binding affinity by up to 20 kcal/mol, representing useful starting points for the development of high affinity biosensors for atrazine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.