ArticleDigestive diseases and sciences2010
Detection of Helicobacter pylori by phenotypic and genotypic methods.
Article in Digestive diseases and sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- Reduction of UreB and CagA expression level by siRNA construct in Helicobacter pylori strain SS1.BMC microbiology · 2023Article
- Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection.World journal of gastroenterology · 2014Article
- Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients.The journal of venomous animals and toxins including tropical diseases · 2014Article
- Agreement rate of rapid urease test, conventional PCR, and scorpion real-time PCR in detecting helicobacter pylori from tonsillar samples of patients with chronic tonsillitis.Journal of global infectious diseases · 2012Article
- State of the globe: Diagnostic tests to detect Helicobacter pylori tonsillitis.Journal of global infectious diseases · 2012Article
- Molecular Basis of pathogenicity in Helicobacter pylori clinical isolates.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsThis research evaluated the utilization of a urease in-house test, culture and molecular method (ureA PCR) as a diagnostic tool for Helicobacter pylori infection. Furthermore, we assessed the presence of the cagA gene in the specimens and in isolated strains that were positive for ureA by PCR positive.
resultsSensitivity and specificity, respectively, were 100 and 95.8% for the urease in-house test 93.3 and 95.8 for the ureA PCR assay of the specimen and 100 and 100% for the culture. The presence of the cagA gene was observed in eight (53%) ureA-positive samples.
conclusionsIn this study, we found that the PCR technique has applicability in the study of cagA, and other genes related to the H. pylori pathogen. This method can be applied to samples directly from biopsy or isolated from the bacteria.
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