ArticleParasitology research2009
In vitro selection of RNA aptamers against a conserved region of the Plasmodium falciparum erythrocyte membrane protein 1.
Article in Parasitology research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Aptamer-Based Biosensors for the Detection of Malaria.Biosensors · 2026Review
- Article
- RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Selection of an Aptamer against the Enzyme 1-deoxy-D-xylulose-5-phosphate Reductoisomerase fromPharmaceutics · 2022Article
- Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application.Biosensors · 2022Review
- Aptamers isolated against mosquito-borne pathogens.World journal of microbiology & biotechnology · 2021Review
- Recent advances in understanding oligonucleotide aptamers and their applications as therapeutic agents.3 Biotech · 2020Review
- High-efficiency enrichment enables identification of aptamers to circulating Plasmodium falciparum-infected erythrocytes.Scientific reports · 2020Article
- Aptamers as a novel diagnostic and therapeutic tool and their potential use in parasitologyBiomedica : revista del Instituto Nacional de Salud · 2020Review
- Therapeutic applications of nucleic acid aptamers in microbial infections.Journal of biomedical science · 2020Review
- Review
- Aptamer Technology: Adjunct Therapy for Malaria.Biomedicines · 2017Review
- Aptamers against pathogenic microorganisms.Critical reviews in microbiology · 2016Review
- Cerebral malaria--clinical manifestations and pathogenesis.Metabolic brain disease · 2016Review
- Molecular and Functional Characterization of ssDNA Aptamers that Specifically Bind Leishmania infantum PABP.PloS one · 2015Article
- In vitro selection of RNA aptamers directed against protein E: a Haemophilus influenzae adhesin.Molecular biotechnology · 2014Article
- Genome-wide identification and functional annotation of Plasmodium falciparum long noncoding RNAs from RNA-seq data.Parasitology research · 2014Article
- Nucleic acid aptamers: research tools in disease diagnostics and therapeutics.BioMed research international · 2014Review
- Aptamers: multifunctional molecules for biomedical research.Journal of molecular medicine (Berlin, Germany) · 2013Review
- RNA aptamers and their therapeutic and diagnostic applications.International journal of biochemistry and molecular biology · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The var-gene encoding Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is known to play a major role in the pathogenicity of the P. falciparum parasite. The protein enables the parasite to adhere to the endothelial linings of small blood vessels (cytoadherence) as well as to non-infected erythrocytes (rosetting), thus preventing clearance from the bloodstream. The development and spread of resistance towards most anti-malarial drugs used for treatment and prevention of the most severe form of malaria truly emphasise the importance of a continuous research and development of new drugs. In this study we use Systematic Evolution of Ligands by EXponential enrichment (SELEX) methodology to isolate high-affinity ligands (aptamers). To validate the results from the SELEX in vitro selection, different aptamers have been selected against PfEMP1 in a live cell assay of P. falciparum strain FCR3S1.2, a highly rosetting strain. We have been able to show the rosette disrupting capacity of these SELEX-aptamers at concentrations of 33 nM and with 100% disruption at 387 nM. The described results show that RNA aptamers are promising candidates for adjunct therapy in severe malaria.
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