ArticleBiomicrofluidics2008
A transparent cell-culture microchamber with a variably controlled concentration gradient generator and flow field rectifier.
Article in Biomicrofluidics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- A Review of In Vitro Instrumentation Platforms for Evaluating Thermal Therapies in Experimental Cell Culture Models.Critical reviews in biomedical engineering · 2022Review
- Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model.Biology · 2021Article
- ECM-based microfluidic gradient generator for tunable surface environment by interstitial flow.Biomicrofluidics · 2020Article
- A nanodroplet cell processing platform facilitating drug synergy evaluations for anti-cancer treatments.Scientific reports · 2019Article
- Doxycycline inhibits electric field-induced migration of non-small cell lung cancer (NSCLC) cells.Scientific reports · 2019Article
- Studying Electrotaxis in Microfluidic Devices.Sensors (Basel, Switzerland) · 2017Review
- Quantifying the roles of random motility and directed motility using advection-diffusion theory for a 3T3 fibroblast cell migration assay stimulated with an electric field.BMC systems biology · 2017Article
- Efficient generation of hepatic cells from mesenchymal stromal cells by an innovative bio-microfluidic cell culture device.Stem cell research & therapy · 2016Article
- Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli.Journal of visualized experiments : JoVE · 2016Article
- Comparison of Chip Inlet Geometry in Microfluidic Devices for Cell Studies.Molecules (Basel, Switzerland) · 2016Article
- Uniform electric field generation in circular multi-well culture plates using polymeric inserts.Scientific reports · 2016Article
- Article
- Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip.Journal of visualized experiments : JoVE · 2015Article
- Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.Biosensors · 2015Article
- Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device.Biomicrofluidics · 2014Article
- Evaluation of EGFR and RTK signaling in the electrotaxis of lung adenocarcinoma cells under direct-current electric field stimulation.PloS one · 2013Article
- Effects of shear stresses and antioxidant concentrations on the production of reactive oxygen species in lung cancer cells.Biomicrofluidics · 2013Article
- A novel method to produce immobilised biomolecular concentration gradients to study cell activities: design and modelling.Molecular biotechnology · 2012Article
- In vitro electrical-stimulated wound-healing chip for studying electric field-assisted wound-healing process.Biomicrofluidics · 2012Article
- Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field.Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Real-time observation of cell growth provides essential information for studies such as cell migration and chemotaxis. A conventional cell incubation device is usually too clumsy for these applications. Here we report a transparent microfluidic device that has an integrated heater and a concentration gradient generator. A piece of indium tin oxide (ITO) coated glass was ablated by our newly developed visible laser-induced backside wet etching (LIBWE) so that transparent heater strips were prepared on the glass substrate. A polymethylmethacrylate (PMMA) microfluidic chamber with flow field rectifiers and a reagent effusion hole was fabricated by a CO(2) laser and then assembled with the ITO heater so that the chamber temperature can be controlled for cell culturing. A variable chemical gradient was generated inside the chamber by combining the lateral medium flow and the flow from the effusion hole. Successful culturing was performed inside the device. Continuous long-term (>10 days) observation on cell growth was achieved. In this work the flow field, medium replacement, and chemical gradient in the microchamber are elaborated.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.