ArticlePloS one2009
Dynamic changes in the spatiotemporal localization of Rab21 in live RAW264 cells during macropinocytosis.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- A new role of RAB21 and VARP in autophagy and autophagic exocytosis of ATP.Autophagy reports · 2025Article
- Cellular Regulation of Macropinocytosis.International journal of molecular sciences · 2024Review
- NHE7 upregulation potentiates the uptake of small extracellular vesicles by enhancing maturation of macropinosomes in hepatocellular carcinoma.Cancer communications (London, England) · 2024Article
- Rab21 regulates caveolin-1-mediated endocytic trafficking to promote immature neurite pruning.EMBO reports · 2023Article
- Functional significance of ion channels during macropinosome resolution in immune cells.Frontiers in physiology · 2022Review
- Macropinocytosis requires Gal-3 in a subset of patient-derived glioblastoma stem cells.Communications biology · 2021Article
- EhRab21 associates with the Golgi apparatus in Entamoeba histolytica.Parasitology research · 2020Article
- The Dual Role of Macropinocytosis in Cancers: Promoting Growth and Inducing Methuosis to Participate in Anticancer Therapies as Targets.Frontiers in oncology · 2020Review
- Endocytic Rabs Are Recruited to theFrontiers in cellular and infection microbiology · 2020Article
- Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.Molecular & cellular proteomics : MCP · 2019Article
- Macropinosomes as units of signal transduction.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy.International journal of nanomedicine · 2018Article
- Mechanistic Insights into the Differential Catalysis by RheB and Its Mutants: Y35A and Y35A-D65A.ACS omega · 2017Article
- Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication ofMolecular & cellular proteomics : MCP · 2017Article
- Vaccinia Virus Infection Requires Maturation of Macropinosomes.Traffic (Copenhagen, Denmark) · 2015Article
- The ancient small GTPase Rab21 functions in intermediate endocytic steps in trypanosomes.Eukaryotic cell · 2014Article
- Small GTPases and phosphoinositides in the regulatory mechanisms of macropinosome formation and maturation.Frontiers in physiology · 2014Review
- Article
- Dissecting the roles of Rac1 activation and deactivation in macropinocytosis using microscopic photo-manipulation.Scientific reports · 2013Article
- Spatiotemporal Localization of Rab20 in Live RAW264 Macrophages during Macropinocytosis.Acta histochemica et cytochemica · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rab21, a member of the Rab GTPase family, is known to be involved in membrane trafficking, but its implication in macropinocytosis is unclear. We analyzed the spatiotemporal localization of Rab21 in M-CSF-stimulated RAW264 macrophages by the live-cell imaging of fluorescent protein-fused Rab21. It was demonstrated that wild-type Rab21 was transiently associated with macropinosomes. Rab21 was recruited to the macropinosomes after a decrease in PI(4,5)P(2) and PI(3,4,5)P(3) levels. Although Rab21 was largely colocalized with Rab5, the recruitment of Rab21 to the macropinosomes lagged a minute behind that of Rab5, and preceded that of Rab7. Then, Rab21 was dissociated from the macropinosomes prior to the accumulation of Lamp1, a late endosomal/lysosomal marker. Our analysis of Rab21 mutants revealed that the GTP-bound mutant, Rab21-Q78L, was recruited to the macropinosomes, similarly to wild-type Rab21. However, the GDP-bound mutant, Rab21-T33N, did not localize on the formed macropinosomes, suggesting that the binding of GTP to Rab21 is required for the proper recruitment of Rab21 onto the macropinosomes. However, neither mutation of Rab21 significantly affected the rate of macropinosome formation. These data indicate that Rab21 is a transient component of early and intermediate stages of macropinocytosis, and probably functions in macropinosome maturation before fusing with lysosomal compartments.
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