ArticlePlant molecular biology2009
Function and regulation of the glutathione peroxidase homologous gene GPXH/GPX5 in Chlamydomonas reinhardtii.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 55 citations in OpenAlex.
- Biochemical and Functional Profiling of Thioredoxin-Dependent Cytosolic GPX-like Proteins inBiomolecules · 2024Article
- Redox regulation, thioredoxins, and glutaredoxins in retrograde signalling and gene transcription.Journal of experimental botany · 2023Review
- Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell.Communications biology · 2023Article
- β-Cyclocitral Does Not Contribute to Singlet Oxygen-Signalling in Algae, but May Down-Regulate Chlorophyll Synthesis.Plants (Basel, Switzerland) · 2022Article
- Selenium-binding Protein 1 (SBD1): A stress response regulator in Chlamydomonas reinhardtii.Plant physiology · 2022Article
- Retrograde and anterograde signaling in the crosstalk between chloroplast and nucleus.Frontiers in plant science · 2022Review
- Chlamydomonas as a model for reactive oxygen species signaling and thiol redox regulation in the green lineage.Plant physiology · 2021Review
- Diverse Biosynthetic Pathways and Protective Functions against Environmental Stress of Antioxidants in Microalgae.Plants (Basel, Switzerland) · 2021Review
- High Carotenoid Mutants ofPlants (Basel, Switzerland) · 2021Article
- Article
- Combined resistance to oxidative stress and reduced antenna size enhance light-to-biomass conversion efficiency inBiotechnology for biofuels · 2019Article
- Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green AlgaFrontiers in plant science · 2017Article
- Genome-wide analysis on Chlamydomonas reinhardtii reveals the impact of hydrogen peroxide on protein stress responses and overlap with other stress transcriptomes.The Plant journal : for cell and molecular biology · 2015Article
- Activation of Autophagy by Metals in Chlamydomonas reinhardtii.Eukaryotic cell · 2015Article
- The regulation of photosynthetic structure and function during nitrogen deprivation in Chlamydomonas reinhardtii.Plant physiology · 2015Article
- Oxidative stress contributes to autophagy induction in response to endoplasmic reticulum stress in Chlamydomonas reinhardtii.Plant physiology · 2014Article
- The PSBP2 protein of Chlamydomonas reinhardtii is required for singlet oxygen-dependent signaling.Planta · 2012Article
- Systematic prediction of cis-regulatory elements in the Chlamydomonas reinhardtii genome using comparative genomics.Plant physiology · 2012Article
- Reactive oxygen species and autophagy in plants and algae.Plant physiology · 2012Review
- SINGLET OXYGEN RESISTANT 1 links reactive electrophile signaling to singlet oxygen acclimation in Chlamydomonas reinhardtii.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When exposed to strong sunlight, photosynthetic organisms encounter photooxidative stress by the increased production of reactive oxygen species causing harmful damages to proteins and membranes. Consequently, a fast and specific induction of defense mechanisms is required to protect the organism from cell death. In Chlamydomonas reinhardtii, the glutathione peroxidase homologous gene GPXH/GPX5 was shown to be specifically upregulated by singlet oxygen formed during high light conditions presumably to prevent the accumulation of lipid hydroperoxides and membrane damage. We now showed that the GPXH protein is a thioredoxin-dependent peroxidase catalyzing the reduction of hydrogen peroxide and organic hydroperoxides.Furthermore, the GPXH gene seems to encode a dual-targeted protein, predicted to be localized both in the chloroplast and the cytoplasm, which is active with either plastidic TRXy or cytosolic TRXh1. Putative dual-targeting is achieved by alternative transcription and translation start sites expressed independently from either a TATA-box or an Initiator core promoter. Expression of both transcripts was upregulated by photooxidative stress even though with different strengths. The induction required the presence of the core promoter sequences and multiple upstream regulatory elements including a Sp1-like element and an earlier identified CRE/AP-1 homologous sequence. This element was further characterized by mutation analysis but could not be confirmed to be a consensus CRE or AP1 element. Instead, it rather seems to be another member of the large group of TGAC-transcription factor binding sites found to be involved in the response of different genes to oxidative stress.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.