ReviewJournal of biomolecular NMR2010
Recent advances in segmental isotope labeling of proteins: NMR applications to large proteins and glycoproteins.
Review in Journal of biomolecular NMR, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Beyond structures: solution NMR as the quantitative engine of integrated structural biology.Frontiers in molecular biosciences · 2026Review
- Solid-state NMR protocols for unveiling dynamics and (drug) interactions of membrane-bound proteins.Protein science : a publication of the Protein Society · 2025Article
- Three segment ligation of a 104 kDa multi-domain protein by SrtA and OaAEP1.Journal of biomolecular NMR · 2023Article
- Primary Structure of Glycans by NMR Spectroscopy.Chemical reviews · 2023Review
- Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy: Advances in Methodology and Applications.Chemical reviews · 2023Review
- Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1.Journal of biomolecular NMR · 2022Article
- Protein resonance assignment by solid-state NMR based onJournal of biomolecular NMR · 2021Article
- Segmental and site-specific isotope labelling strategies for structural analysis of posttranslationally modified proteins.RSC chemical biology · 2021Review
- Virus Structures and Dynamics by Magic-Angle Spinning NMR.Annual review of virology · 2021Review
- Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling.Biomolecules · 2020Article
- Review
- Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation.Journal of biomolecular NMR · 2018Article
- A molecular engineering toolbox for the structural biologist.Quarterly reviews of biophysics · 2017Review
- Natural Inhibitors of Snake Venom Metalloendopeptidases: History and Current Challenges.Toxins · 2016Review
- Characterizing Aciniform Silk Repetitive Domain Backbone Dynamics and Hydrodynamic Modularity.International journal of molecular sciences · 2016Article
- Segmental Isotope Labelling of an Individual Bromodomain of a Tandem Domain BRD4 Using Sortase A.PloS one · 2016Article
- Efficient segmental isotope labeling of multi-domain proteins using Sortase A.Journal of biomolecular NMR · 2015Article
- BCL::SAXS: GPU accelerated Debye method for computation of small angle X-ray scattering profiles.Proteins · 2015Article
- Chemical ligation of the influenza M2 protein for solid-state NMR characterization of the cytoplasmic domain.Protein science : a publication of the Protein Society · 2015Article
- Spider wrapping silk fibre architecture arising from its modular soluble protein precursor.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the last 15 years substantial advances have been made to place isotope labels in native and glycosylated proteins for NMR studies and structure determination. Key developments include segmental isotope labeling using Native Chemical Ligation, Expressed Protein Ligation and Protein Trans-Splicing. These advances are pushing the size limit of NMR spectroscopy further making larger proteins accessible for this technique. It is just emerging that segmental isotope labeling can be used to define inter-domain interactions in NMR structure determination. Labeling of post-translational modified proteins like glycoproteins remains difficult but some promising developments were recently achieved. Key achievements are segmental and site-specific labeling schemes that improve resonance assignment and structure determination of the glycan moiety. We adjusted the focus of this perspective article to concentrate on the NMR applications based on recent developments rather than on labeling methods themselves to illustrate the considerable potential for biomolecular NMR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.