ArticleHistochemistry and cell biology2009
Relocalization of a microtubule-anchoring protein, ninein, from the centrosome to dendrites during differentiation of mouse neurons.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Architects of the Developing Brain: Cytoskeleton-Organizing Molecules in Neurodevelopmental Disorders.Cells · 2026Review
- Ninein promotes F-actin cup formation and inward phagosome movement during phagocytosis in macrophages.Molecular biology of the cell · 2024Article
- An alternative splice isoform of mouse CDK5RAP2 induced cytoplasmic microtubule nucleation.IBRO neuroscience reports · 2022Article
- Microtubule Anchoring: Attaching Dynamic Polymers to Cellular Structures.Frontiers in cell and developmental biology · 2022Review
- Article
- Distinct Microtubule Organizing Center Mechanisms Combine to Generate Neuron Polarity and Arbor Complexity.Frontiers in cellular neuroscience · 2020Review
- Epidermal development requires ninein for spindle orientation and cortical microtubule organization.Life science alliance · 2019Article
- Ninein is essential for apico-basal microtubule formation and CLIP-170 facilitates its redeployment to non-centrosomal microtubule organizing centres.Open biology · 2017Article
- Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex.Cell · 2016Article
- Loss of γ-tubulin, GCP-WD/NEDD1 and CDK5RAP2 from the Centrosome of Neurons in Developing Mouse Cerebral and Cerebellar Cortex.Acta histochemica et cytochemica · 2015Article
- mNos2 deletion and human NOS2 replacement in Alzheimer disease models.Journal of neuropathology and experimental neurology · 2014Article
- Longitudinal study of differential protein expression in an Alzheimer's mouse model lacking inducible nitric oxide synthase.Journal of proteome research · 2013Article
- Ninein is essential for the maintenance of the cortical progenitor character by anchoring the centrosome to microtubules.Biology open · 2013Article
- Spindle orientation and epidermal morphogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis.The Journal of cell biology · 2011Article
- A PAR-1-dependent orientation gradient of dynamic microtubules directs posterior cargo transport in the Drosophila oocyte.The Journal of cell biology · 2011Article
- The DISC1 Ser704Cys substitution affects centrosomal localization of its binding partner PCM1 in glia in human brain.Human molecular genetics · 2010Article
- Extending the knowledge in histochemistry and cell biology.Histochemistry and cell biology · 2010Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules in typical cells form radial arrays with their plus-ends pointing toward the cell periphery. In contrast, microtubules in dendrites of neurons are free from centrosomes and have a unique arrangement in which about half have a polarity with a minus-end distal orientation. Mechanisms for generation and maintenance of the microtubule arrangement in dendrites are not well understood. Here, we examined dendritic localization of a centrosomal protein, ninein, which has microtubule-anchoring and stabilizing functions. Immunohistochemical analysis of developing mouse cerebral and cerebellar cortices showed that ninein is localized at the centrosome in undifferentiated neural precursors. In contrast, ninein was barely detected in migrating neurons, such as those in the intermediate layer of the cerebral cortex and the internal granular layer of the cerebellar cortex. High expression was observed in thick dendrite-bearing neurons such as pyramidal neurons of the cerebral cortex and Purkinje neurons in the cerebellar cortex. Ninein was not detected at the centrosome of these cells, but was diffusely present in cell soma and dendrites. In cultured cortical neurons, ninein formed granular structures in soma and dendrites, being not associated with gamma-tubulin. About 60% of these structures showed resistance to detergent and association with microtubules. Our observations suggest that the minus-ends of microtubules may be anchored and stabilized by centrosomal proteins localized in dendrites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.