ArticlePlant cell reports2009
Regulation of seed germination and seedling growth by an Arabidopsis phytocystatin isoform, AtCYS6.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- Article
- Phytocystatin 6 is a context-dependent, tight-binding inhibitor of Arabidopsis thaliana legumain isoform β.The Plant journal : for cell and molecular biology · 2023Article
- Maquiberry Cystatins: Recombinant Expression, Characterization, and Use to Protect Tooth Dentin and Enamel.Biomedicines · 2023Article
- Proteomic Analysis of Embryo Isolated From MatureFrontiers in plant science · 2022Article
- CRISPR/Cas9 Guided Mutagenesis ofInternational journal of molecular sciences · 2021Article
- Article
- Quantitative proteomic analyses of two soybean low phytic acid mutants to identify the genes associated with seed field emergence.BMC plant biology · 2019Article
- VOZ1, a transcriptional repressor of DREB2C, mediates heat stress responses in Arabidopsis.Planta · 2018Article
- N-terminomics reveals control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway.The New phytologist · 2018Article
- TheMolecules and cells · 2017Article
- Defense-related proteins involved in sugarcane responses to biotic stress.Genetics and molecular biology · 2017Article
- Rice bifunctional phytocystatin is a dual modulator of legumain and papain-like proteases.Plant molecular biology · 2016Article
- Cereal cystatins delay sprouting and nutrient loss in tubers of potato, Solanum tuberosum.BMC plant biology · 2015Article
- Genome-wide identification and characterization of cystatin family genes in rice (Oryza sativa L.).Plant cell reports · 2015Article
- Subfamily-Specific Fluorescent Probes for Cysteine Proteases Display Dynamic Protease Activities during Seed Germination.Plant physiology · 2015Article
- The diversity of rice phytocystatins.Molecular genetics and genomics : MGG · 2014Article
- Comprehensive analysis of cystatin family genes suggests their putative functions in sexual reproduction, embryogenesis, and seed formation.Journal of experimental botany · 2014Article
- A novel Glycine soja cysteine proteinase inhibitor GsCPI14, interacting with the calcium/calmodulin-binding receptor-like kinase GsCBRLK, regulated plant tolerance to alkali stress.Plant molecular biology · 2014Article
- DREB2C acts as a transcriptional activator of the thermo tolerance-related phytocystatin 4 (AtCYS4) gene.Transgenic research · 2014Article
- Identification of BZR1-interacting proteins as potential components of the brassinosteroid signaling pathway in Arabidopsis through tandem affinity purification.Molecular & cellular proteomics : MCP · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytocystatins are cysteine proteinase inhibitors in plants that are implicated in the endogenous regulation of protein turnover and defense mechanisms against insects and pathogens. A cDNA encoding a phytocystatin called AtCYS6 (Arabidopsis thaliana phytocystatin6) has been isolated. We show that AtCYS6 is highly expressed in dry seeds and seedlings and that it also accumulates in flowers. The persistence of AtCYS6 protein expression in seedlings was promoted by abscisic acid (ABA), a seed germination and post-germination inhibitory phytohormone. This finding was made in transgenic plants bearing an AtCYS6 promoter-beta-glucuronidase (GUS) reporter construct, where we found that expression from the AtCYS6 promoter persisted after ABA treatment but was reduced under control conditions and by gibberellin(4+7) (GA(4+7)) treatment during the germination and post-germinative periods. In addition, constitutive over-expression of AtCYS6 retarded germination and seedling growth, whereas these were enhanced in an AtCYS6 knock-out mutant (cys6-2). Additionally, cysteine proteinase activities stored in seeds were inhibited by AtCYS6 in transgenic Arabidopsis. From these data, we propose that AtCYS6 expression is enhanced by the germination inhibitory phytohormone ABA and that it participates in the control of germination rate and seedling growth by inhibiting the activity of stored cysteine proteinases.
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