ArticleMolecular systems biology2009
Quantifying epistatic interactions among the components constituting the protein translation system.
Article in Molecular systems biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Elucidating the Bell-Shaped Dependence of Protein Translation Activity on EF-Tu Concentration in a Reconstituted Cell-Free System Using a Mechanistic Model.ACS synthetic biology · 2026Article
- A universal polyphosphate kinase powers in vitro transcription.Nature communications · 2026Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- On biochemical constructors and synthetic cells.Interface focus · 2023Article
- The Long Road to a Synthetic Self-Replicating Central Dogma.Biochemistry · 2023Review
- A versatile active learning workflow for optimization of genetic and metabolic networks.Nature communications · 2022Article
- Evolutionary transition from a single RNA replicator to a multiple replicator network.Nature communications · 2022Article
- Cell-Free PURE System: Evolution and Achievements.Biodesign research · 2022Review
- Epistasis shapes the fitness landscape of an allosteric specificity switch.Nature communications · 2021Article
- In vitro synthesis of 32 translation-factor proteins from a single template reveals impaired ribosomal processivity.Scientific reports · 2021Article
- A partially self-regenerating synthetic cell.Nature communications · 2020Article
- Strategies for in vitro engineering of the translation machinery.Nucleic acids research · 2020Review
- Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology.Frontiers in bioengineering and biotechnology · 2020Review
- Extrinsic stochastic factors (solute partition) in gene expression inside lipid vesicles and lipid-stabilized water-in-oil droplets: a review.Synthetic biology (Oxford, England) · 2018Review
- Synthetic microbial consortia enable rapid assembly of pure translation machinery.Nature chemical biology · 2018Article
- Article
- Mini-review:Computational and structural biotechnology journal · 2017Review
- In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes.Scientific reports · 2016Article
- Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system inBiochemistry and biophysics reports · 2015Article
- A transcription and translation-coupled DNA replication system using rolling-circle replication.Scientific reports · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In principle, the accumulation of knowledge regarding the molecular basis of biological systems should allow the development of large-scale kinetic models of their functions. However, the development of such models requires vast numbers of parameters, which are difficult to obtain in practice. Here, we used an in vitro translation system, consisting of 69 defined components, to quantify the epistatic interactions among changes in component concentrations through Bahadur expansion, thereby obtaining a coarse-grained model of protein synthesis activity. Analyses of the data measured using various combinations of component concentrations indicated that the contributions of larger than 2-body inter-component epistatic interactions are negligible, despite the presence of larger than 2-body physical interactions. These findings allowed the prediction of protein synthesis activity at various combinations of component concentrations from a small number of samples, the principle of which is applicable to analysis and optimization of other biological systems. Moreover, the average ratio of 2- to 1-body terms was estimated to be as small as 0.1, implying high adaptability and evolvability of the protein translation system.
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