ArticlePlant & cell physiology2009
Resolving the role of plant glutamate dehydrogenase. I. In vivo real time nuclear magnetic resonance spectroscopy experiments.
Article in Plant & cell physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 116 citations in OpenAlex.
- Foliar application of licorice-wolfberry derived nanomaterials enhances soybean heat tolerance through maintaining reactive oxygen species homeostasis.Frontiers in plant science · 2026Article
- Proline Tagging for Stress Tolerance in Plants.International journal of genomics · 2025Review
- Glutamate dehydrogenase from Pantoea ananatis: A new bacterial enzyme with dual coenzyme specificity.PloS one · 2025Article
- ArabidopsisPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Genome-wide assessment of genetic diversity and transcript variations in 17 accessions of the model diatomISME communications · 2024Article
- Article
- Reversible Parahydrogen Induced Hyperpolarization ofAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Plants (Basel, Switzerland) · 2023Article
- The diversity of substrates for plant respiration and how to optimize their use.Plant physiology · 2023Review
- Polyamines Metabolism Interacts with γ-Aminobutyric Acid, Proline and Nitrogen Metabolisms to Affect Drought Tolerance of Creeping Bentgrass.International journal of molecular sciences · 2022Article
- Nitrogen Uptake and Distribution in Different Chinese Cabbage Genotypes under Low Nitrogen Stress.International journal of molecular sciences · 2022Article
- Nitrogen metabolic rate and differential ammonia volatilization regulate resistance against opportunistic fungusFrontiers in plant science · 2022Article
- Source-Sink Dynamics in Field-Grown Durum Wheat Under Contrasting Nitrogen Supplies: Key Role of Non-Foliar Organs During Grain Filling.Frontiers in plant science · 2022Article
- Glutamate Dehydrogenase Functions in Glutamic Acid Metabolism and Stress Resistance inMolecules (Basel, Switzerland) · 2021Article
- Histopathological, hematological, and biochemical changes in high-latitude fish Phoxinus lagowskii exposed to hypoxia.Fish physiology and biochemistry · 2021Article
- The phosphorylated pathway of serine biosynthesis links plant growth with nitrogen metabolism.Plant physiology · 2021Article
- Expression of cyanobacterial genes enhanced COPeerJ · 2021Article
- Glutamate dehydrogenase mediated amino acid metabolism after ammonium uptake enhances rice growth under aeration condition.Plant cell reports · 2020Article
- NADH-GOGAT Overexpression Does Not Improve Maize (Plants (Basel, Switzerland) · 2020Article
- Structural Studies of Glutamate Dehydrogenase (Isoform 1) FromFrontiers in plant science · 2020Article
Corrections and comments
- Erratum issuedMoshou, Panagiotis N [corrected to Moschou, Panagiotis N]
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher plants the glutamate dehydrogenase (GDH) enzyme catalyzes the reversible amination of 2-oxoglutarate to form glutamate, using ammonium as a substrate. For a better understanding of the physiological function of GDH either in ammonium assimilation or in the supply of 2-oxoglutarate, we used transgenic tobacco (Nicotiana tabacum L.) plants overexpressing the two genes encoding the enzyme. An in vivo real time (15)N-nuclear magnetic resonance (NMR) spectroscopy approach allowed the demonstration that, when the two GDH genes were overexpressed individually or simultaneously, the transgenic plant leaves did not synthesize glutamate in the presence of ammonium when glutamine synthetase (GS) was inhibited. In contrast we confirmed that the primary function of GDH is to deaminate Glu. When the two GDH unlabeled substrates ammonium and Glu were provided simultaneously with either [(15)N]Glu or (15)NH(4)(+) respectively, we found that the ammonium released from the deamination of Glu was reassimilated by the enzyme GS, suggesting the occurrence of a futile cycle recycling both ammonium and Glu. Taken together, these results strongly suggest that the GDH enzyme, in conjunction with NADH-GOGAT, contributes to the control of leaf Glu homeostasis, an amino acid that plays a central signaling and metabolic role at the interface of the carbon and nitrogen assimilatory pathways. Thus, in vivo NMR spectroscopy appears to be an attractive technique to follow the flux of metabolites in both normal and genetically modified plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.