Evidence map›Paper›PMID 19689323›Full record

ArticleCurrent pharmaceutical biotechnology2009

Single-quantum dot imaging with a photon counting camera.

X Michalet, R A Colyer, J Antelman, O H W Siegmund, A Tremsin, J V Vallerga, S Weiss

Abstract read
In one paragraph

Article in Current pharmaceutical biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Wide-field time-correlated single photon counting-based fluorescence lifetime imaging microscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2019
    Review
  3. Article
  4. Development of new photon-counting detectors for single-molecule fluorescence microscopy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013
    Review
  5. Article
  6. New photon-counting detectors for single-molecule fluorescence spectroscopy and imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2011
    Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

X MichaletDepartment of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA. michalet@chem.ucla.edu
R A Colyer
J Antelman
O H W Siegmund
A Tremsin
J V Vallerga
S Weiss

Funding

High-Resolution, High Speed, High-Throughput 3-Dimensional Detector for BiologyR01EB006353 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2007 to 2010
$1.6M
Multipixel Hybrid Photon-Counting Detector for High-Throughput Single-Molecule AsR01GM084327 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2008 to 2010
$667k
NIBIB NIH HHS R01 EB006353NIGMS NIH HHS 5R01 GM-084327NIGMS NIH HHS R01 GM084327
6 · The paper itself

Abstract

The expanding spectrum of applications of single-molecule fluorescence imaging ranges from fundamental in vitro studies of biomolecular activity to tracking of receptors in live cells. The success of these assays has relied on progress in organic and non-organic fluorescent probe developments as well as improvements in the sensitivity of light detectors. We describe a new type of detector developed with the specific goal of ultra-sensitive single-molecule imaging. It is a wide-field, photon-counting detector providing high temporal and high spatial resolution information for each incoming photon. It can be used as a standard low-light level camera, but also allows access to a lot more information, such as fluorescence lifetime and spatio-temporal correlations. We illustrate the single-molecule imaging performance of our current prototype using quantum dots and discuss on-going and future developments of this detector.

Indexed as

PhotonsQuantum DotsElectronsEquipment DesignMicroscopy, FluorescenceNanotechnologyRadiographic Image Interpretation, Computer-AssistedSpectrometry, Fluorescence

Identifiers

PMID19689323
PMCPMC2830710

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.