ArticleThe Journal of cell biology2009
Steric and not structure-specific factors dictate the endocytic mechanism of glycosylphosphatidylinositol-anchored proteins.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- A live cell imaging-based assay for tracking particle uptake by clathrin-mediated endocytosis.Methods in enzymology · 2024Article
- Selective Endocytic Uptake of Targeted Liposomes Occurs within a Narrow Range of Liposome Diameters.ACS applied materials & interfaces · 2023Article
- Caveolar and non-Caveolar Caveolin-1 in ocular homeostasis and disease.Progress in retinal and eye research · 2022Review
- Cargo crowding contributes to sorting stringency in COPII vesicles.The Journal of cell biology · 2020Article
- Molecular thermodynamics of receptor competition for endocytic uptake.Soft matter · 2019Article
- Receptor Heterodimerization Modulates Endocytosis through Collaborative and Competitive Mechanisms.Biophysical journal · 2019Article
- BAR scaffolds drive membrane fission by crowding disordered domains.The Journal of cell biology · 2019Article
- Structure Versus Stochasticity-The Role of Molecular Crowding and Intrinsic Disorder in Membrane Fission.Journal of molecular biology · 2018Review
- The 2018 biomembrane curvature and remodeling roadmap.Journal of physics D: Applied physics · 2018Article
- Folate action in nervous system development and disease.Developmental neurobiology · 2018Review
- Bigger Isn't Always Better: Bulking Up Impedes Receptor Internalization.Biophysical journal · 2018Article
- Entropic Control of Receptor Recycling Using Engineered Ligands.Biophysical journal · 2018Article
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane.Nature communications · 2015Article
- Article
- Clathrin-independent pathways of endocytosis.Cold Spring Harbor perspectives in biology · 2014Article
- Endocytosis of gene delivery vectors: from clathrin-dependent to lipid raft-mediated endocytosis.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Review
- The non-peptidic part determines the internalization mechanism and intracellular trafficking of peptide amphiphiles.PloS one · 2013Article
- Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1.Developmental cell · 2012Article
- LRP1 mediates Hedgehog-induced endocytosis of the GPC3-Hedgehog complex.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diverse glycosylphosphatidylinositol (GPI)-anchored proteins enter mammalian cells via the clathrin- and dynamin-independent, Arf1-regulated GPI-enriched early endosomal compartment/clathrin-independent carrier endocytic pathway. To characterize the determinants of GPI protein targeting to this pathway, we have used fluorescence microscopic analyses to compare the internalization of artificial lipid-anchored proteins, endogenous membrane proteins, and membrane lipid markers in Chinese hamster ovary cells. Soluble proteins, anchored to cell-inserted saturated or unsaturated phosphatidylethanolamine (PE)-polyethyleneglycols (PEGs), closely resemble the GPI-anchored folate receptor but differ markedly from the transferrin receptor, membrane lipid markers, and even protein-free PE-PEGs, both in their distribution in peripheral endocytic vesicles and in the manner in which their endocytic uptake responds to manipulations of cellular Arf1 or dynamin activity. These findings suggest that the distinctive endocytic targeting of GPI proteins requires neither biospecific recognition of their GPI anchors nor affinity for ordered-lipid microdomains but is determined by a more fundamental property, the steric bulk of the lipid-anchored protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.