ArticleInfection and immunity2009
The membrane localization domain is required for intracellular localization and autoregulation of YopE in Yersinia pseudotuberculosis.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Role of the Yersinia pseudotuberculosis Virulence Plasmid in Pathogen-Phagocyte Interactions in Mesenteric Lymph Nodes.EcoSal Plus · 2021Review
- Structural insight into the membrane targeting domain of the Legionella deAMPylase SidD.PLoS pathogens · 2020Article
- VopE, aFrontiers in cellular and infection microbiology · 2017Article
- Yersinia pseudotuberculosis Blocks Neutrophil Degranulation.Infection and immunity · 2016Article
- A bacterial type III secretion-based protein delivery tool for broad applications in cell biology.The Journal of cell biology · 2015Article
- Colonization of cecum is important for development of persistent infection by Yersinia pseudotuberculosis.Infection and immunity · 2014Article
- The GAP activity of type III effector YopE triggers killing of Yersinia in macrophages.PLoS pathogens · 2014Article
- Effector CD8+ T cells are generated in response to an immunodominant epitope in type III effector YopE during primary Yersinia pseudotuberculosis infection.Infection and immunity · 2014Article
- Yersinia pseudotuberculosis efficiently escapes polymorphonuclear neutrophils during early infection.Infection and immunity · 2014Article
- Article
- Regulation of the Yersinia type III secretion system: traffic control.Frontiers in cellular and infection microbiology · 2013Review
- Domain analyses reveal that Chlamydia trachomatis CT694 protein belongs to the membrane-localized family of type III effector proteins.The Journal of biological chemistry · 2012Article
- A protective epitope in type III effector YopE is a major CD8 T cell antigen during primary infection with Yersinia pseudotuberculosis.Infection and immunity · 2012Article
- Bacterial toxin effector-membrane targeting: outside in, then back again.Frontiers in cellular and infection microbiology · 2012Review
- Imaging of bubonic plague dynamics by in vivo tracking of bioluminescent Yersinia pestis.PloS one · 2012Article
- Destabilization of YopE by the ubiquitin-proteasome pathway fine-tunes Yop delivery into host cells and facilitates systemic spread of Yersinia enterocolitica in host lymphoid tissue.Infection and immunity · 2011Article
- The RACK1 signaling scaffold protein selectively interacts with Yersinia pseudotuberculosis virulence function.PloS one · 2011Article
- A solvent-exposed patch in chaperone-bound YopE is required for translocation by the type III secretion system.Journal of bacteriology · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent work has shown that a domain of YopE of Yersinia pseudotuberculosis ranging from amino acids 54 to 75 (R. Krall, Y. Zhang, and J. T. Barbieri, J. Biol. Chem. 279:2747-2753, 2004) is required for proper localization of YopE after ectopic expression in eukaryotic cells. This domain, called the membrane localization domain (MLD), has not been extensively studied in Yersinia. Therefore, an in cis MLD deletion mutant of YopE was created in Y. pseudotuberculosis. The mutant was found to secrete and translocate YopE at wild-type levels. However, the mutant was defective in the autoregulation of YopE expression after the infection of HeLa cells. Although the mutant translocated YopE at wild-type levels, it showed a delayed HeLa cell cytotoxicity. This delay was not caused by a change in GTPase activating protein (GAP) activity, since the mutant showed wild-type YopE GAP activity toward Rac1 and RhoA. The MLD mutant displayed a changed intracellular localization pattern of YopE in HeLa cells after infection, and the YopEDeltaMLD protein was found to be dispersed within the whole cell, including the nucleus. In contrast, wild-type YopE was found to localize to the perinuclear region of the cell and was not found in the nucleus. In addition, the yopEDeltaMLD mutant was avirulent. Our results suggest that YopE must target proteins other than RhoA and Rac1 and that the MLD is required for the proper targeting and hence virulence of YopE during infection. Our results raise the question whether YopE is a regulatory protein or a "true" virulence effector protein.
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